FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757021

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757021
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences194447
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGTTA152.0838565E-4126.94025470
AACACGA250.001587832776.1641670
TAGCCCG150.00233829569.1060936
CGTTACA150.002359918668.94591529
TTGCACG200.006684547853.00626866
GGATCGT200.007253265551.9233938
GTATCAA6200.045.581531
GTGGTAT901.8189894E-1242.122771
CACGGTT400.001037941441.55805669
TCAACGC7350.038.4101684
CAACGCA7400.038.1506425
ATCAACG7400.038.1506423
TATCAAC7500.038.1010132
AACGCAG7450.037.89466
GGTATCA2100.037.7463761
ACGCAGA8050.035.0701527
TGGTATC1102.910383E-1134.4286232
AGAGTAC8550.033.01926411
CGCAGAG8550.033.0192648
CAGAGTA8700.032.4499710
Produced by FastQC (version 0.11.5)