FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757023

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757023
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences255540
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT17360.6793456993034358No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC13750.5380762307270877No Hit
GGGCAGTGGTGGGTGCTTTATTTCCATGCTGGGTGCCCGGGAAGTATGTA10960.4288956719104641No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC7440.29114815684432965No Hit
GGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCCGGGGAGCGGGGGCTTGCCGGCCGTCGCACTCATTTA6070.23753619785552163No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG5660.22149174297565938No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG5190.20309931908898804No Hit
GTGGTGGGTGCTTTATTTCCATGCTGGGTGCCCGGGAAGTATGTAGACGG4970.19449009939735462No Hit
CTTTATTTCCATGCTGGGTGCCCGGGAAGTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAG4740.1854895515379197No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG4460.17453236283947718No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT4390.17179306566486657No Hit
ATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTT4370.1710104093292635RNA PCR Primer, Index 33 (95% over 22bp)
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT4370.1710104093292635No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCTATAGCAAGCTCACCGTGGACAAGAG4270.16709712765124834No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT4050.15848790795961493No Hit
CTCATGCTCCGTGATGCATGAGGCTCTGCACAACCACTACACGCAGAAGA3540.1385301714017375No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT3260.12757298270329498No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT3230.12639899819989045No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC3170.12405102919308131No Hit
TCCCAGGGCAGTGGTGGGTGCTTTATTTCCATGCTGGGTGCCCGGGAAGT3070.12013774751506615No Hit
ACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCT2950.1154418095014479RNA PCR Primer, Index 33 (95% over 24bp)
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC2930.11465915316584488No Hit
CCACTACACGCAGAAGAGCCTCTCCCTGTCCCCGGGTAAATGAGTGCGACGGCCGGCAAGCCCCCGCTCCCCGGG2920.11426782499804336No Hit
CGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCAT2910.11387649683024186No Hit
GTGCCAAGCATCCTCGTGCGACCGCGAGAGCCCGGGGAGCGGGGGCTTGC2840.11113719965563122No Hit
CTGCACAACCACTACACGCAGAAGAGCCTCTCCCTGTCCCCGGGTAAATGAGTGCGACGGCCGGCAAGCCCCCG2840.11113719965563122No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC2830.11074587148782969No Hit
GCATGAGGCTCTGCACAACCACTACACGCAGAAGAGCCTCTCCCTGTCCC2810.10996321515222666No Hit
GTGCTGGACTCCGACGGCTCCTTCTTCCTCTATAGCAAGCTCACCGTGGA2760.10800657431321906No Hit
GCTTTATTTCCATGCTGGGTGCCCGGGAAGTATGTAGACGGGGTACGTGC2700.10565860530640996No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCT2660.10409329263520388No Hit
GCAGTGGTGGGTGCTTTATTTCCATGCTGGGTGCCCGGGAAGTATGTAGA2660.10409329263520388No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG2590.10135399546059325No Hit
CGCATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCATAGGTTCTCAACCCTCACCCCCCAC2580.10096266729279174No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT2560.10018001095718869No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTTAG303.1492964E-584.2798270
TAAGCGC208.103638E-569.67065447
ACGTAAA307.640023E-659.2271362
CACGTAA252.2256814E-456.80032361
GACGTTA302.4413163E-455.74318369
GGCAACG200.006540204453.3044262
CTTATAC3050.051.623111
TATACAC3050.051.602913
AACACGT200.00759179451.33250417
TTATACA3100.050.7706032
AGTACCC305.719202E-446.91446755
GTATCAA8500.046.3089641
CTATATA306.5651594E-445.6199573
TATACCG306.5651594E-445.6199576
ATACCGC306.5651594E-445.6199577
AGGGGGG3050.045.59400670
ATACACA3750.041.9703674
TACACAT3900.041.2334255
AAAGACG350.001095336641.11428566
TCTAGCA504.0558352E-641.0579643
Produced by FastQC (version 0.11.5)