FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757024

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757024
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences255540
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG42671.6697972920090787No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC12460.4875948970806919No Hit
GGGCAGTGGTGGGTGCTTTATTTCCATGCTGGGTGCCCGGGAAGTATGTA11800.4617672380057916No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC6970.27275573295765826No Hit
GGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCCGGGGAGCGGGGGCTTGCCGGCCGTCGCACTCATTTA6340.24810205838616262No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG5730.22423104015027No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG5560.21757846129764422No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG4690.18353291069891212No Hit
GTGGTGGGTGCTTTATTTCCATGCTGGGTGCCCGGGAAGTATGTAGACGG4680.1831415825311106No Hit
CTTTATTTCCATGCTGGGTGCCCGGGAAGTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAG4610.18040228535649996No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT4580.1792283008530954No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT4460.17453236283947718No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCTATAGCAAGCTCACCGTGGACAAGAG4280.16748845581904986No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC3780.1479220474289739No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT3570.13970415590514204No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT3210.12561634186428738No Hit
CTCATGCTCCGTGATGCATGAGGCTCTGCACAACCACTACACGCAGAAGA3180.12444235736088283No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT3030.11857243484386007No Hit
CTGCACAACCACTACACGCAGAAGAGCCTCTCCCTGTCCCCGGGTAAATGAGTGCGACGGCCGGCAAGCCCCCG3010.11778977850825702No Hit
CCACTACACGCAGAAGAGCCTCTCCCTGTCCCCGGGTAAATGAGTGCGACGGCCGGCAAGCCCCCGCTCCCCGGG2970.11622446583705096No Hit
GCATGAGGCTCTGCACAACCACTACACGCAGAAGAGCCTCTCCCTGTCCC2910.11387649683024186No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA2870.11231118415903577No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG2860.11191985599123425No Hit
TCCCAGGGCAGTGGTGGGTGCTTTATTTCCATGCTGGGTGCCCGGGAAGT2850.11152852782343273No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT2850.11152852782343273No Hit
GTGCTGGACTCCGACGGCTCCTTCTTCCTCTATAGCAAGCTCACCGTGGA2850.11152852782343273No Hit
GTGCCAAGCATCCTCGTGCGACCGCGAGAGCCCGGGGAGCGGGGGCTTGC2730.10683258980981451No Hit
GAGCAGGTGGCAGCAGGGGAACGTCTTCTCATGCTCCGTGATGCATGAGG2710.10604993347421147No Hit
TTTCCATGCTGGGTGCCCGGGAAGTATGTAGACGGGGTACGTGCCAAGCA2710.10604993347421147No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC2670.1044846208030054No Hit
CGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCAT2600.10174532362839477No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATTCGG150.002197601870.20193554
GTATTTC150.00224349869.83710550
TAGTCGT150.002434265668.4149259
AGGCTCG300.002664110266.8570670
TAGTGTG200.0067315652.91725558
CGTATTT200.007010000852.37782349
CGGGTAT200.00761015551.30114417
CTCATAT200.00762194551.2810672
GCGCACT306.1159825E-446.28055243
CACGTCC306.1159825E-446.28055243
TAGCGCA306.17009E-446.19793741
CATAGCG306.2611134E-446.06090539
TTACTGC306.534283E-445.66359729
GGTATCA3900.042.0850071
ACGGGTC350.001403381139.0942416
TATAATT350.001407439139.071294
ACGGGAC557.815348E-637.31722616
GTATCAA8700.036.9453161
GGGCAGT3350.036.7458651
ATAGCGC400.002558311734.59356340
Produced by FastQC (version 0.11.5)