FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757026

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757026
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2113718
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACGGCCGTGTCGGCGGCAGTCACAGAGTACAGCTTCAGGGAGA27470.1299605718454401No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT26530.12551343178229074No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT21910.10365621147191821No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC21840.10332504146721559No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA39800.038.270991
TATCAAC46000.033.1033252
GTGGTAT7350.032.8201941
ATCAACG47250.031.7900963
TCAACGC47250.031.644274
CAACGCA47850.031.2474785
AACGCAG49250.030.4313376
TAATGAT12950.030.4230770
CGCAGAG56250.026.6537848
ACGCAGA56450.026.5562067
GGTATCA29000.026.1429821
GCAGAGT59650.025.3668639
AGTACGG49600.023.07168813
AGAGTAC65500.022.89132511
CAGAGTA66900.022.46380410
GAGTACG51250.022.26163712
TTAACGG2151.364242E-1020.89634335
AGTACTT17400.020.60188913
GAGTACT17600.020.36777912
CTAATGA13050.020.3380869
Produced by FastQC (version 0.11.5)