FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757028

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757028
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1822141
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG26130.14340273337793288No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA63900.041.351551
GGTATCA24950.039.0615731
TATCAAC72600.036.3912052
ATCAACG75900.034.7176133
TCAACGC75950.034.6495824
CAACGCA76900.034.2661485
AACGCAG78250.033.7673036
ACGCAGA81250.032.5330127
CGCAGAG83100.031.610888
AGAGTAC83800.031.30671111
CAGAGTA84550.031.15084610
GCAGAGT88900.029.7038469
GTGGTAT11000.029.324941
AGTACGG65250.029.26054413
GAGTACG65550.029.07424212
CGTTACG500.007181544328.02382555
TACGGGG27700.026.899515
GTACGGG72000.026.18351614
TGGTATC12600.024.780682
TACGGGA21600.024.16318115
Produced by FastQC (version 0.11.5)