FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757029

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757029
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1084463
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTATGAGCTGACTCAGCCACTCTCAGTGTCAGTGGCCCTGGGACAGACG19150.17658509326735905No Hit
GGCCTGGACCGCTCTCCTTCTGAGCCTCCTTGCTCACTTTACAGGTTCTG18820.17354211254786933No Hit
CTTTACAGGTTCTGTGGCCTCCTATGAGCTGACTCAGCCACTCTCAGTGT18780.17317326640005237No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC15000.13831730543135173No Hit
GCCATGGCCTGGACCGCTCTCCTTCTGAGCCTCCTTGCTCACTTTACAGGTTCTGTGGCCTCCTATGAGCTGACT14640.1349976901009993No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATGCCAC14340.13223134399237224No Hit
CCCCTGTGCTGGTCATCAATAGGGATAAGAATCGGCCCTCTGGGATCCCA13710.1264220171642555No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATGCCACGCTGCTGTCCCACACCTGACAGTAATAGTCAGCCT13670.12605317101643854No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT13220.12190365185349801No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG12210.1125902866211203No Hit
GTCCCACACCTGACAGTAATAGTCAGCCTCATCCCCGGCTTGGGCTCTGCTGATGGTCAGGGTGGCCGTATTCCC12210.1125902866211203No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG11990.11056163280812716No Hit
CAATTACCTGTGGGGGATACAACATTGAAAGTAAAAATGTGCACTGGTACCAGCAGAAGCCAGGCCAGGCCC11870.10945509436467635No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC11770.10853297899513399No Hit
CTCTCAGTGTCAGTGGCCCTGGGACAGACGGCCACAATTACCTGTGGGGGATACAACATTGAAAGTAAAAATGTG11220.1034613444626511No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC11020.10161711372356642No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC10950.10097163296488677No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10700.040.974391
GTATCAA26200.040.0557441
TATCAAC29900.034.388822
TCAACGC31050.033.2262844
ATCAACG31150.033.1196173
CAACGCA31550.032.5903555
AACGCAG31800.032.3341456
GTGGTAT4700.031.5837061
ACGCAGA33800.030.522967
AGAGTAC34650.029.8765411
TGGTATC4750.029.782592
CGCAGAG35100.029.4921468
CAGAGTA36400.028.62976610
TAATTAG1004.8427864E-727.71197941
GCAGAGT37850.027.4405549
AGTACGG28300.025.85006713
GAGTACG28450.025.59248412
TACGGGG14200.025.03001615
ACTAGCG851.4384234E-424.36490627
TATGTAC4150.023.2799552
Produced by FastQC (version 0.11.5)