FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757030

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757030
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1084463
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCCTGGACCGCTCTCCTTCTGAGCCTCCTTGCTCACTTTACAGGTTCTG17360.16007922815255107No Hit
CCTATGAGCTGACTCAGCCACTCTCAGTGTCAGTGGCCCTGGGACAGACG17280.1593415358569172No Hit
CTTTACAGGTTCTGTGGCCTCCTATGAGCTGACTCAGCCACTCTCAGTGT16200.14938268986585987No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATGCCAC16130.14873720910718022No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC15790.14560201685073626No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATGCCACGCTGCTGTCCCACACCTGACAGTAATAGTCAGCCT15650.14431105533337696No Hit
GCCATGGCCTGGACCGCTCTCCTTCTGAGCCTCCTTGCTCACTTTACAGGTTCTGTGGCCTCCTATGAGCTGACT14430.13306124782496037No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG14380.1326001901401892No Hit
CCCCTGTGCTGGTCATCAATAGGGATAAGAATCGGCCCTCTGGGATCCCA12920.11913730574487096No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC12190.11240586354721185No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT11860.10936288282772211No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11840.10917845975381363No Hit
GTCCCACACCTGACAGTAATAGTCAGCCTCATCCCCGGCTTGGGCTCTGCTGATGGTCAGGGTGGCCGTATTCCC11810.10890182514295094No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC11240.10364576753655956No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG11030.10170932526052065No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12450.040.966631
TAGGTCG350.001356374439.3776445
GTATCAA28900.038.396881
TATCAAC31350.035.279732
ATCAACG32500.033.819343
TCAACGC32400.033.6046834
TGGTATC5050.033.4320072
CAACGCA32800.033.4049645
AACGCAG33550.032.763936
GTGGTAT5500.031.9554821
ACGCAGA34950.031.4631047
AGAGTAC36200.030.77176511
CGCAGAG35650.030.761398
CAGAGTA35900.030.74071510
GCAGAGT37850.029.0645229
AGTACGG31400.027.45799413
GAGTACG32250.026.94816812
GTACGGG35700.023.95752114
TACGGGA16700.023.7432215
CGGGATA2200.023.49559617
Produced by FastQC (version 0.11.5)