FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757032

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757032
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1726165
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC72680.42104897272276987No Hit
ATATTATACTACGCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT60910.3528631388077038No Hit
GCTTAGGAGGCTGTCCTGGTTTCCGCTGGTACCAAGCTAAGTAGTTCTTATTGTTGGAGTTGGAGCTGTATAAAA57050.33050142946937283No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT56510.32737310743758563No Hit
CTCCTAAGCTGCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCT51120.2961478190091909No Hit
GTTTTATACAGCTCCAACTCCAACAATAAGAACTACTTAGCTTGGTACCAGCGGAAACCAGGACAGCCTCCTAAG49720.28803735448233514No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC44310.2566962022749853No Hit
ATACTACGCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGA37170.21533283318802085No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG36200.20971343990869937No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC35810.2074540962190752No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC35210.20397818285042274No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT35120.2034567958451249No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC33910.19644703721834242No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC32490.18822070891253154No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC31880.1846868636544015No Hit
GTATAAAACACTCTGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA31740.18387581720171595No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT29940.17344807709575852No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACGCGCTGCCA27930.16180376731077273No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG27240.15780646693682238No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT26690.15462021301555762No Hit
GCGTAGTATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAAT26280.15224500554697842No Hit
ATTCAGTGGCAGCGCGTCTGGGACAGATTTCACTCTCACCATCAGCAGCC23910.13851514774080115No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC23710.13735650995125032No Hit
GTCCCTGACCGATTCAGTGGCAGCGCGTCTGGGACAGATTTCACTCTCAC23600.13671925916699737No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT23280.13486543870371603No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT23190.13434405169841815No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT22960.13301161824043473No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT22950.1329536863509572No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTATA22810.1321426398982716No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG22660.13127366155610848No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA22600.13092607021924324No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG22550.13063641077185553No Hit
GTAATACACGGCCGTGTCCCCGGCTCTCAGACTGTTCATTTGCAGAGACA22410.12982536431916994No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT22300.129188113534917No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG22170.12843499897170896No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG21260.1231631970292527No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA20830.12067212578171843No Hit
GTCAGGGACCCCGGATTCCCGGGTAGATGCCCAGTAAATGAGCAGCTTAGGAGGCTGTCCTGGTTTCCGCTGGTA20320.11771759941836384No Hit
TCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACGCGCTGCCACTGAATCGGTCAGGGACC20160.11679068918672318No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA19930.11545825572873972No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG19720.11424168604971136No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19320.1119244104706097No Hit
AACCAGGACAGCCTCCTAAGCTGCTCATTTACTGGGCATCTACCCGGGAA19180.11111336401792413No Hit
GTTTATTACTGTCAGCAATATTATACTACGCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA18630.10792711009665935No Hit
GAGTGAAATCTGTCCCAGACGCGCTGCCACTGAATCGGTCAGGGACCCCG18430.10676847230710854No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA18120.10497258373330476No Hit
GTAAATGAGCAGCTTAGGAGGCTGTCCTGGTTTCCGCTGGTACCAAGCTA17940.103929809722709No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC17480.10126494280674211No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC17470.10120701091726457No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA17410.10085941958039933No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA17280.10010630501719128No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAGCG200.007461038451.571235
TGGTATC5050.044.252542
GTGGTAT5150.044.0685121
GTATCAA32650.039.494791
GGTATCA13450.039.372241
TATCAAC36800.034.9413762
ATCAACG38250.033.5269133
TCAACGC38550.033.0876354
CAACGCA39350.032.414955
AACGCAG39400.032.3747526
CGCAGAG42850.029.8760838
AGAGTAC43500.029.19316911
CAGAGTA44650.028.59542710
ACGCAGA45000.028.3606857
GCAGAGT46250.027.680599
GAGTACG41150.025.17333812
AGTACGG41100.025.12022813
CTGTATA19150.024.54457566
GTATAGG1151.7477414E-623.9212471
TATAAAA22900.023.88019669
Produced by FastQC (version 0.11.5)