FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757037

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757037
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences598716
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6850.039.291861
GTATCAA16550.038.363511
CGATACG400.002425271834.98000360
TATCAAC19100.033.0306472
TCAACGC19150.032.944414
ATCAACG19400.032.697573
CAACGCA19350.032.6038975
AACGCAG19900.031.7027846
TACGTCG450.0046376130.64415
ACGCAGA21550.029.2754257
AGAGTAC21750.029.00622611
CAGAGTA21650.028.98096810
CGCAGAG22300.028.445428
GTGGTAT2200.028.2324351
GCAGAGT23000.027.729589
TAACCCG600.00801488227.39664869
AGTACGG18750.025.1894513
GAGTACG18900.024.98953412
TGGTATC2600.023.8670392
TACGGGC5300.023.41671815
Produced by FastQC (version 0.11.5)