FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005757039

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757039
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2455416
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCT80640.32841685482215643No Hit
GTAATATACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTCCAAATACA76780.31269650438052043No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGTTTTGGAGATGGTTTT71170.2898490520547231No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGAGCGCCTGAGT64230.26158500229696313No Hit
GTATATTACTGTGCGAAGCATTGGTACGGATTGGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGC63520.25869343524681765No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGCCTTTTT59700.2431359899911054No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT59240.24126258035298298No Hit
ACCACATACTACGCAGACTCCGTGAAGGGCCGCTTCACCATCTCCAGAGA52760.21487194023334538No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGACCTCCAGCAACTTCGGCACCCAGAC51380.20925171131897813No Hit
GCCCAGGGCCGCTGTGCTCTCGGAGGTGCTCCTGGAGCAGGGCGCCAGGG51080.20802992242455048No Hit
GTGGTGGACGTGAGCCACGAAGACCCCGAGGTCCAGTTCAACTGGTACGT50710.20652304945475633No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCTGGGGAGCGGGGGCTTGCTGGCCGTGGCACTCATTTA45830.18664861677206632No Hit
GACCTACACCTGCAACGTAGATCACAAGCCCAGCAACACCAAGGTGGACAAGACAGTTGAGCGCAAATGTTGTGT41880.17056172966210206No Hit
GCATGGAGGTGCATAATGCCAAGACAAAGCCACGGGAGGAGCAGTTCAACAGCACGTTCCGTGTGGTCAGCGTC39740.1618463022151847No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT39100.1592398192404057No Hit
CCGCTGGTCAGAGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA38550.15699987293395498No Hit
GTATGTGGTACCACCACTACCACTAATTTTTGAGACCCACTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAACT38190.1555337262606418No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGGCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAACCAAAGGGCAG37870.15423048477325227No Hit
CCTCCATGCCGTCCACGTACCAGTTGAACTGGACCTCGGGGTCTTCGTGGCTCACGTCCACCACCACGCACGTGA37440.15247925402457263No Hit
ATATTGGATCCAGCTTGCAAACCGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCA37400.15231634883864895No Hit
GATCAGGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGCC36650.14926187660257978No Hit
GTCCCACTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG36340.1479993614116712No Hit
CTATTATGCTGTAGACAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATTGTGAGAGTGAATTCTGTC35680.1453114258439303No Hit
GTGTGAGGTGCAGCTGTTGGAGTCTGGAGGAGGCTTCATACAGCCTGGGGGGTCCCTGAGAATCTCCTGTGCAGC35320.14384527917061712No Hit
GAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGCCTTTTTCTTGT33240.1353742095025853No Hit
CACCAGGACTGGCTGAACGGCAAGGAGTACAAGTGCAAGGTCTCCAACAA32590.13272700023132536No Hit
GCCTGGGGGGTCCCTGAGAATCTCCTGTGCAGCCTCTGGATTCACCTTTAACAACTATGCCATGAGTTGGGTCCG32580.13268627393484445No Hit
GCATAATAGTTACCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT31970.1302019698495082No Hit
GTGTAGGTCTGGGTGCCGAAGTTGCTGGAGGTCACGGTCACCACGCTGCT31750.1293059913269279No Hit
GTCTTCCCCCTGGCGCCCTGCTCCAGGAGCACCTCCGAGAGCACAGCGGCCCTGGGCTGCCTGGTCAAGGACTA31260.1273104027993627No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC31010.12629224538733966No Hit
CTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAACTCATGGCATAGTTGT29720.12103855314130069No Hit
GTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT29240.11908369091021645No Hit
CTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCACGACGGTGAGGACGCTGACCACACGGAACGTGCTGTTGAA28760.11712882867913217No Hit
GTCTCCAACAAAGGCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAAC28700.11688447090024663No Hit
GGCTTCATACAGCCTGGGGGGTCCCTGAGAATCTCCTGTGCAGCCTCTGG28670.11676229201080388No Hit
GTCCTGGTGCACGACGGTGAGGACGCTGACCACACGGAACGTGCTGTTGA28130.11456307200083408No Hit
CAACTGTCTTGTCCACCTTGGTGTTGCTGGGCTTGTGATCTACGTTGCAGGTGTAGGTCTGGGTGCCGAAGTTG27990.11399290385010116No Hit
GTGCTGGGCACGGTGGGCACTCGACACAACATTTGCGCTCAACTGTCTTG27660.11264893606623073No Hit
GCCACAGGTGGTGCTGGGCACGGTGGGCACTCGACACAACATTTGCGCTCAACTGTCTTGTCCACCTTGGTGTTG27230.11089770531755108No Hit
GGGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGTTTTGGAGATGGTT27040.11012390568441355No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT26840.10930937975479511No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC26810.10918720086535234No Hit
GTGTAGTGGTTGTGCAGAGCCTCATGCATCACGGAGCATGAGAAGACGTT26750.1089428430864668No Hit
CTGAGAGAGGAGCCCAGCCCTGGGATTTTCAGGTGTTTTCATTTGGTGAT26550.10812831715684837No Hit
GGGCTGGAGTGGGTCTCAAAAATTAGTGGTAGTGGTGGTACCACATACTA26500.10792468567444376No Hit
GTATCAACGCAGAGTACGGGAGCTCTGAGAGAGGAGCCCAGCCCTGGGAT26480.10784323308148192No Hit
GTTCAACAGCACGTTCCGTGTGGTCAGCGTCCTCACCGTCGTGCACCAGG25910.10552183418206937No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACGTGCGTGGTGGTGGACGTG25460.10368915084042786No Hit
GTCCTTGACCAGGCAGCCCAGGGCCGCTGTGCTCTCGGAGGTGCTCCTGG25450.10364842454394693No Hit
CCTTTAACAACTATGCCATGAGTTGGGTCCGCCAGGCTCCAGGGAAGGGG24840.10116412045861067No Hit
CTACGCAGACTCCGTGAAGGGCCGCTTCACCATCTCCAGAGACAATTCCAAGAACACGTTGTATTTGGAAATGAA24770.10087903638324423No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC24750.10079758379028239No Hit
GAGGTGCATAATGCCAAGACAAAGCCACGGGAGGAGCAGTTCAACAGCAC24700.10059395230787778No Hit
CAGTAATATACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTCCAAATA24560.10002378415714486No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)