FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005757041

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757041
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1379522
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA26930.19521254463502577No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT26790.1941977003628793No Hit
GTAATATACGGCCGTGTCCGCGGCAGTAAGAGACCTCAGTTTCAGGGAGA24440.1771628143661355No Hit
GTCCCAGACCCACTGGCACTGAACCTGGCTGGGATACCAGTGGCCCTGGT24110.1747706814389332No Hit
GTATAAAAACTGGCCTCGGACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT20690.14997948564792732No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG19280.13975855404988105No Hit
GTCTGGGACAGAGTTCACTCTCAGCATCAGCAGCCTGCAGTCTGAAGATT18920.13714895449293307No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC17480.1267105562651411No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTATCAGCAACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCT17470.1266380673885592No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTC16630.12054900175568059No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16420.11902673534746093No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC16230.11764944669240505No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT15640.11337260297407362No Hit
CTGGTATCCCAGCCAGGTTCAGTGCCAGTGGGTCTGGGACAGAGTTCACT15630.11330011409749173No Hit
TCTATGATGCGTCCACCAGGGCCACTGGTATCCCAGCCAGGTTCAGTGCCAGTGGGTCTGGGACAGAGTTCACT15470.11214029207218153No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT15380.11148789218294454No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG15040.10902327037916032No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC14930.10822589273675955No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC14850.10764598172410444No Hit
GTTTTTATACTGTTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGGCTGCTGATGCTGAGAGTGAACT14680.10641367082221233No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT14460.10481891553741078No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT14280.10351411575893678No Hit
GAGTTCACTCTCAGCATCAGCAGCCTGCAGTCTGAAGATTTTGCAGTTTATTACTGTCAACAGTATAAAAACTGG14140.10249927148679036No Hit
CATCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGCTAAGTTGCTGATAACACTCTGA13950.10112198283173447No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)