FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757042

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757042
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1379522
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT26840.19456014474578875No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA25980.18832610135974634No Hit
GTCCCAGACCCACTGGCACTGAACCTGGCTGGGATACCAGTGGCCCTGGT20680.14990699677134542No Hit
GTAATATACGGCCGTGTCCGCGGCAGTAAGAGACCTCAGTTTCAGGGAGA20390.14780481935047066No Hit
GTATAAAAACTGGCCTCGGACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT19790.14345548675555736No Hit
GTCTGGGACAGAGTTCACTCTCAGCATCAGCAGCCTGCAGTCTGAAGATT18970.13751139887584252No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG17980.13033500009423554No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTC16870.12228873479364591No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC16620.1204765128790987No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT15990.11590971365443974No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTATCAGCAACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCT15950.11561975814811218No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT15930.1154747803949484No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT15700.11380753623356496No Hit
GTTTTTATACTGTTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGGCTGCTGATGCTGAGAGTGAACT15610.11315513634432796No Hit
CTGGTATCCCAGCCAGGTTCAGTGCCAGTGGGTCTGGGACAGAGTTCACT15110.10953069251523352No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC14400.10438398227791945No Hit
GAGTTCACTCTCAGCATCAGCAGCCTGCAGTCTGAAGATTTTGCAGTTTATTACTGTCAACAGTATAAAAACTGG14100.1022093159804628No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC14060.10191936047413526No Hit
TCTATGATGCGTCCACCAGGGCCACTGGTATCCCAGCCAGGTTCAGTGCCAGTGGGTCTGGGACAGAGTTCACT13840.1003246051893337No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12000.040.197321
GTATCAA28100.039.2368351
TATCAAC31300.034.6674652
ATCAACG32450.033.3327263
TCAACGC32700.033.077894
CAACGCA33150.032.628875
AACGCAG34150.031.7788856
TACTGAT9000.031.30299270
CGCAGAG37400.029.0299828
AGAGTAC37250.028.96181911
ACGCAGA38200.028.509157
GCAGAGT40300.027.02659
CAGAGTA39950.027.0044510
TTCGGGG9350.025.2261470
AGTACGG38100.024.24474313
GAGTACG38800.023.89617312
GTGGTAT7550.022.817781
CTTAGAC7550.022.17925558
CGAACTA1253.514122E-622.07270829
GACGTGT7900.021.69795862
Produced by FastQC (version 0.11.5)