FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757043

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757043
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences542151
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC19790.3650274554506032No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC19320.3563582839467233No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18070.33330197675555334No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG17550.3237105529640266No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT16770.3093234172767365No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC13900.2563861359658103No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG13590.2506681717824001No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC12980.23941669387310915No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG12960.23904779295805043No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG11680.21543813439429235No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT11070.20418665648500142No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA10940.20178880053711973No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10430.19238182720312239No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT10190.18795501622241775No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG10110.18647941256218287No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG9650.1779946915158323No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT9500.1752279346528919No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG9100.1678499163517175No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT9000.16600541177642392No Hit
GGTAATCTCCCTCCCTTCACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT8860.16342310537101287No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG8790.16213195216830734No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG8740.16120969988066056No Hit
GCTTAGGGGCTTTCCCTGGTTTCTGTTGATACCAATTTAAATAGTAACTG8730.16102524942313118No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG8440.15567618615477977No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT8270.15254052837678064No Hit
CCTCATCTACGATGCATCCAATTTGGAAACAGGGGTCCCATCAAGGTTCA7840.14460915870301816No Hit
ATATTACTGTCAACAGTCAGGTAATCTCCCTCCCTTCACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA7710.1422113027551365No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC7660.14128905046748969No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG7500.1383378431470199No Hit
CAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACAT7460.13760004131690248No Hit
CATCTACGATGCATCCAATTTGGAAACAGGGGTCCCATCAAGGTTCAGTG7390.13630888811419697No Hit
ACATATTACTGTCAACAGTCAGGTAATCTCCCTCCCTTCACTTTTGGCCA7350.13557108628407954No Hit
CCCCTAAGCTCCTCATCTACGATGCATCCAATTTGGAAACAGGGGTCCCA7240.13354213125125658No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC7110.13114427530337489No Hit
AACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTC7020.12948422118561065No Hit
CTGATGTCCTGACTCGCCTGGCAAGTGATGGTGACTCTCTCTCCTACAGA6890.12708636523772895No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT6860.1265330138651409No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC6670.12302845517208306No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC6660.1228440047145537No Hit
TCGTAGATGAGGAGCTTAGGGGCTTTCCCTGGTTTCTGTTGATACCAATT6660.1228440047145537No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC6510.12007724785161329No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG6490.11970834693655458No Hit
CCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCT6460.1191549955639665No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT6460.1191549955639665No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC6410.11823274327631969No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGAGAGAGTCACCATCACTTGCCAGGCGAGTCAGGACATCAGTTA6320.11657268915855545No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA6200.11435928366820312No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA6120.11288368000796827No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA6070.11196142772032147No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT5970.11011692314502786No Hit
GGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCT5860.1080879681122049No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA5840.10771906719714618No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT5780.10661236445197002No Hit
ACCTTGATGGGACCCCTGTTTCCAAATTGGATGCATCGTAGATGAGGAGC5750.10605901307938195No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG5650.10421450850408834No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA5630.10384560758902964No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT5570.10273890484385348No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGCG150.002315011369.29767643
GTGGTAT1050.046.0406721
ATCAACG9750.035.740373
TCAACGC9900.035.198854
AGTATTA400.002593276334.50502815
TTAGACT400.002594449834.5018464
ACGTAGA400.002594449834.5018462
CAACGCA10200.034.163595
TGTTAGA6400.032.82796570
AACGCAG10800.032.2656146
AGAGTAC11150.030.9462111
CAGAGTA11650.029.61804610
CGCAGAG12200.028.8458048
GCAGAGT12700.027.981819
GAGCAAA11200.027.85402970
ACGCAGA12850.027.3866777
TATTCAT2400.027.336651
GTATCAA13550.027.0127941
TACGGGC2350.026.42938215
AGTACGG11700.026.24741413
Produced by FastQC (version 0.11.5)