FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005757045

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757045
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1408982
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCTAGGACGGTCACCTTGGTCCCTCCGCCGAACACCTTAGTGCCACTACCTGCATATGAGCAGCAGTAATAAT58310.4138448894308089No Hit
GCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTCCGCCGAACACCTTAGTGCCACTACCTGCATATGAGCAG55450.3935465463717776No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC47330.33591628565872383No Hit
GTACCAACACCACCCAGGCAAAGCCCCCAAACTCATGATTTATGAGGACA44240.31398555836767256No Hit
CCTTAGTGCCACTACCTGCATATGAGCAGCAGTAATAATCAGCCTCGTCC43430.308236726941863No Hit
GTGTTGGTACCAGGAGACAAGGTTATAACTCCCAACACCACTGCTGGTTCCAGTGCAGGAGATGGTGATCGA41290.29304845626132914No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT39510.2804152217700439No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG38770.27516320293658825No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGTGGTGTT36840.2614653700331161No Hit
GTCCTGGGCCCAGTCTGCCCTTACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG34320.24358011670837526No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG33010.23428262390860918No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG32090.22775308698052918No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC32060.22754016729809182No Hit
GTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATTGTCAGGGAGG31650.22463026497144747No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT28690.20362218963762488No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG28390.201492992813251No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTC28070.1992218495339188No Hit
CTCCTGCACTGGAACCAGCAGTGGTGTTGGGAGTTATAACCTTGTCTCCTGGTACCAACACCACCCAGGCAAAGC27890.1979443314392945No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT26070.18502720403809275No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC26050.18488525758313448No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG25280.17942031906724146No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA24980.17729112224286753No Hit
GGACACAGGGTCCTGGGCCCAGTCTGCCCTTACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC24920.17686528287799277No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGCAGTGGTGTTGGGAGTTATAACCTTGTCTCCTGGTACCAACACCA24070.17083255854226667No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG23570.16728389716831016No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC22350.15862516341585628No Hit
ACCTAGGACGGTCACCTTGGTCCCTCCGCCGAACACCTTAGTGCCACTAC22010.15621207368156584No Hit
GCCTGGAGCCCAGAGATTGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCC21770.15450871622206672No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC21650.15365703749231713No Hit
GATTATTACTGCTGCTCATATGCAGGTAGTGGCACTAAGGTGTTCGGCGGAGGGACCAAGGTGACCGTCCTAGGT21640.15358606426483803No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG21630.1535150910373589No Hit
GCACTAAGGTGTTCGGCGGAGGGACCAAGGTGACCGTCCTAGGTCAGCCC21470.1523795193976928No Hit
CTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTTACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGA21160.15017934934583976No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA20880.1481920989764241No Hit
ACCTGCATATGAGCAGCAGTAATAATCAGCCTCGTCCTCAGCCTGGAGCC20710.1469855541092789No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA20530.14570803601465454No Hit
GTGATACACAGCCGTGTCCTCAGCTCTCAGGCTGTTCATTTGCAGATACA19820.1406689368636363No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC19780.14038504395371978No Hit
CCTTGGTCCCTCCGCCGAACACCTTAGTGCCACTACCTGCATATGAGCAGCAGTAATAATCAGCCTCGTCCTCAG19320.13712027548967978No Hit
GGCCTGGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTTACTCAGC18990.13477815898286846No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA18960.1345652393004311No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG18760.1331457747508485No Hit
GTTATAACCTTGTCTCCTGGTACCAACACCACCCAGGCAAAGCCCCCAAA18640.13229409602109893No Hit
CCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAACATTCTGTAGGGGCCA18430.13080365824403717No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG18350.13023587242420415No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC18340.13016489919672503No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT18080.12831959528226763No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT17250.1224288174014998No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGC17060.12108032607939634No Hit
GCCCTTACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC17020.12079643316947981No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC16700.11852528989014764No Hit
CCCTTACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACC16620.11795750407031459No Hit
GTGTTGGGAGTTATAACCTTGTCTCCTGGTACCAACACCACCCAGGCAAA16380.11625414661081547No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT16280.11554441433602416No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA15980.11341521751165025No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT15920.11298937814677547No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACC15740.11171186005215113No Hit
GTTATTGTCCTCATAAATCATGAGTTTGGGGGCTTTGCCTGGGTGGTGTTGGTACCAGGAGACAAGGTTATAACT15090.10709860026600765No Hit
TATGCAGGTAGTGGCACTAAGGTGTTCGGCGGAGGGACCAAGGTGACCGTCCTAGGTCAGCCCAAGGCTGCCCC15060.10688568058357026No Hit
GTGTATCACTGTGCGAGAGAATTAGGATATTGTGGTGATGGTACCTGCTC15000.10645984121869549No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTCCGCCGAACACCTTAGTGCCA14950.10610497508129982No Hit
GCTCTAGTCTCCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAACATTCT14950.10610497508129982No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT14930.10596302862634158No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC14930.10596302862634158No Hit
CGTCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGC14740.10461453730423809No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT14730.10454356407675898No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG14680.10418869793936332No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG14300.10149171529515637No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG14220.10092392947532332No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT14200.10078198302036505No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGG14150.1004271168829694No Hit
ACCATGGCCTGGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTTACT14120.10021419720053203No Hit
CTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTC14110.10014322397305289No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)