FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757049

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757049
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1043330
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA35550.043.135451
GGTATCA13700.042.1626361
TATCAAC40250.037.6479262
ATCAACG41100.036.702113
TCAACGC41350.036.563314
CAACGCA41550.036.3873145
AACGCAG42800.035.32466
ACGCAGA45900.032.9372677
CGCAGAG46200.032.650588
AGAGTAC46150.032.5401611
CAGAGTA47000.031.95167210
GCAGAGT49350.030.8464899
AGTACGG34650.029.7528113
GAGTACG34900.029.53968212
TACCGTT1103.7523932E-828.15689729
TACGGGC7850.027.14008515
ACGCGTA907.0694605E-626.75230627
GTACGGG38950.026.2034814
TTAACGG1501.036824E-925.30981335
TACGGGT5300.024.63751415
Produced by FastQC (version 0.11.5)