FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757050

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757050
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1043330
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11100.10639011626235229No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA38200.042.303121
GGTATCA15100.040.444361
TATCAAC42850.037.3031852
ATCAACG43400.036.6723753
TCAACGC43650.036.540924
CAACGCA44000.036.1740345
CTTACGA700.002217429535.62454270
AACGCAG45000.035.2956286
CAGAGTA47750.033.28842510
ACGCAGA47700.033.1730087
AGAGTAC48150.032.72668511
CGCAGAG48400.032.6979378
GCAGAGT50850.031.3265579
GCGTACG450.004749375430.4973681
AGTACGG38750.030.29981813
GAGTACG39300.029.78841812
GCCGATA500.00709117828.0954757
CCGTTAT500.00773850627.59712635
GTACGGG42550.027.5938414
TTCGACG500.0079222327.4647489
Produced by FastQC (version 0.11.5)