FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757053

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757053
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1329072
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATAGCGGCTGCAC16080.12098667340821265No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGAT13310.10014506362334019No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA42700.041.954671
GGTATCA16350.041.6406861
TCGTATG1700.038.66775540
ATCAACG49450.036.334033
TCAACGC49500.036.1585244
CAACGCA49800.036.009695
AACGCAG51250.035.191976
ACGCAGA53250.033.8056957
CTCGTAT1450.033.391839
TATCAAC53500.033.390872
GACGGAC2200.032.81103526
CGCAGAG54800.032.7880558
AGAGTAC55050.032.76396611
CAGAGTA56250.032.12608310
GCAGAGT59050.030.4863839
AGTACGG45900.028.6668913
TATCTCG1700.028.41666636
GAGTACG46400.028.28394112
TAGTTAG1554.847432E-628.08098470
GTGGTAT6300.027.8355671
Produced by FastQC (version 0.11.5)