FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757055

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757055
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1778695
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCGC38520.2165632668894892No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC35690.20065272573431647No Hit
GTGTGGGGGCTGCTCCCGGGAGTTGTTGGTACCAGGATACATAATTATTG35070.19716702413848353No Hit
ACATATGATACCAGCCTGAGTGCTGCGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGG32750.18412375365085076No Hit
GCCCCCACACTCCTCATTTTTGACAATACTAAGCGACCCTCAGGGATTCCTGACCGATTCTCTGGCTCCAAGT32690.18378642768996373No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT31970.17973851615931905No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCGCAGCACTCAGG30450.17119292515018034No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG28530.16039849440179457No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG26400.14842342279030413No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC25970.14600592007061355No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC24640.1385285279376172No Hit
ATCATATGTTGCGCAATAGTAGTCGGCCTCGTCCCCAGTCTGGAGTCCGG23880.1342557324330478No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC23220.13054514686329022No Hit
GTCCAGGGTGGCTGACGTGCCAGACTTGGAGCCAGAGAATCGGTCAGGAA22980.129195843019742No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG22460.12627235135872086No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT22460.12627235135872086No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT22460.12627235135872086No Hit
ATATGATACCAGCCTGAGTGCTGCGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGC22370.12576636241739025No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG21600.12143734591933975No Hit
ACCCTGGACATCACCGGACTCCAGACTGGGGACGAGGCCGACTACTATTG20670.1162087935255904No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT20600.11581524657122216No Hit
GTATCATATGTTGCGCAATAGTAGTCGGCCTCGTCCCCAGTCTGGAGTCCGGTGATGTCCAGGGTGGCTGACGTG20550.11553414160381627No Hit
ATACTAAGCGACCCTCAGGGATTCCTGACCGATTCTCTGGCTCCAAGTCTGGCACGTCAGCCACCCTGGACATCA20480.11514059464944805No Hit
GGTCAGAGGGTCACCGTCTCCTGCTCTGGAAGCACCTCCAACATTGGCAATAATTATGTATCCTGGTACCAACAA20340.1143535007407116No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC20340.1143535007407116No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA18910.10631389867290346No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT18690.10507703681631758No Hit
ATAGTAGTCGGCCTCGTCCCCAGTCTGGAGTCCGGTGATGTCCAGGGTGG17970.10102912528567293No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC17930.10080424131174823No Hit
CCCTCAGTGTCTGCGGCCCCAGGTCAGAGGGTCACCGTCTCCTGCTCTGG17930.10080424131174823No Hit
GTATCAACGCAGAGTACGGGAAGCTCTGCTTCAGCTGTGAGCGCAGAAGG17840.10029825237041763No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA39100.038.8708041
GTGGTAT5450.038.486761
TATCAAC45250.033.413272
ATCAACG46450.032.6980173
TCAACGC46700.032.5229764
CAACGCA47050.032.2080085
AACGCAG48250.031.406986
AGAGTAC52000.029.33947211
CGCAGAG52250.029.2640328
CAGAGTA53050.028.75876810
ACGCAGA52900.028.708797
GCGAACG500.00784074727.52405529
TATACAC3000.026.3445913
TCGTATG1359.262294E-925.59981240
ACGAGAC2150.025.57497622
CGTATGC1901.8189894E-1225.4830941
AGTACGG44850.024.43998513
GCAGAGT63300.024.1555429
GAGTACG46400.023.69761712
GAGTACT18000.023.67128612
Produced by FastQC (version 0.11.5)