FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757057

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757057
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences462444
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTATACTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGCGAGAGTGAATTCTGTC20270.4383233429344958No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC18670.40372455908174826No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC18450.39896722630199544No Hit
GTATAATACTTATTCTCCGTGGACATTCGGCCAAGGGACCAAGGTGGAAGTCAAACGAACTGTGGCTGCACCAT17890.38685765195353383No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT15830.3423117177431213No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15570.3366894153670499No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG14770.319390023440676No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC13360.28889984517044226No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC11800.2551660309140134No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG11290.2441376685609501No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC11270.24370518376279077No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG10850.23462300300144448No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG10560.22835197342813401No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT10560.22835197342813401No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA10070.21775609587323003No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT10020.21667488387783168No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT9580.20716021831832612No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9530.20607900632292775No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT9360.20240288553857333No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG9200.19894300715329857No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG9190.19872676475421888No Hit
TACTTATTCTCCGTGGACATTCGGCCAAGGGACCAAGGTGGAAGTCAAACGAACTGTGGCTGCACCATCTGTCTT9150.1978617951579002No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT8920.19288821997906774No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG8640.18683343280483689No Hit
GTAATACTCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTGCTACAGA8640.18683343280483689No Hit
GAGTATTACTAACTATTTGGCCTGGTATCAACAGAAACCAGGGAAAGCCCCTAAACTCCTGATCTATAAGACGT8320.1799136760342874No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG8180.17688628244717197No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG8000.17299391926373786No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA7910.17104773767202083No Hit
CCTTCCACCCTGTCTGCATCTGTAGCAGACAGAGTCACCATCACTTGCCG7840.16953404087846313No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT7820.16910155608030378No Hit
GAATTCACTCTCGCCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTA7640.16520919289686967No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC7610.16456046569963065No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT7510.16239804170883393No Hit
GGACCAAGGTGGAAGTCAAACGAACTGTGGCTGCACCATCTGTCTTCATC7410.1602356177180372No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG7060.15266713375024868No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC6990.15115343695669098No Hit
GTTTAGGGGCTTTCCCTGGTTTCTGTTGATACCAGGCCAAATAGTTAGTA6930.14985598256221294No Hit
GTCTGCATCTGTAGCAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGA6880.1487747705668146No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG6850.14812604336957555No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC6800.1470448313741772No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG6500.14055755940178702No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA6490.14034131700270736No Hit
GTGCAGCCACAGTTCGTTTGACTTCCACCTTGGTCCCTTGGCCGAATGTC6490.14034131700270736No Hit
GGTATCAACAGAAACCAGGGAAAGCCCCTAAACTCCTGATCTATAAGACGTCTAGTTTAGAAAATGGGGTCCCAT6340.1370976810165123No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC6340.1370976810165123No Hit
GTTGATACCAGGCCAAATAGTTAGTAATACTCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTGCTACAGATG6190.13385404503031717No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC6180.1336378026312375No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC6020.13017792424596275No Hit
ACCTTGATGGGACCCCATTTTCTAAACTAGACGTCTTATAGATCAGGAGT5920.12801550025516603No Hit
ATAATACACAGCCGTGTCCTCGGGAGTCACAGAGTTCAGCTGCAGGGAGA5910.12779925785608637No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT5880.12715053065884735No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG5830.12606931866344898No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT5810.12563683386528965No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT5770.12477186426897095No Hit
GTAGCAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATTACTAACTATTTGGCCTGGTATCAACAGAAA5720.1236906522735726No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA5670.12260944027817422No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA5650.12217695548001486No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT5630.12174447068185554No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA5570.1204470162873775No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT5530.1195820466910588No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGTCTCCTTCCACC5520.11936580429197914No Hit
GTCTTATAGATCAGGAGTTTAGGGGCTTTCCCTGGTTTCTGTTGATACCAGGCCAAATAGTTAGTAATACTCTGA5440.11763586509934175No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA5410.11698713790210274No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC5320.1150409563103857No Hit
CTCCGGGGACAGTGTCTCTAGCACCAGTGCTGCTTGGAACTGGATCAGGC5310.11482471391130601No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT5280.11417598671406701No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT5190.11222980512234995No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG5160.11158107792511092No Hit
CATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCGCCATCAGCAGCCTGCAGCCTGATGATTTTG5120.11071610832879225No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT5120.11071610832879225No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG5110.11049986592971256No Hit
GATCAGGAGTTTAGGGGCTTTCCCTGGTTTCTGTTGATACCAGGCCAAAT5070.10963489633339389No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT4990.1079049571407565No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT4970.10747247234259717No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGACTTCCACCTTGGTCC4960.10725622994351748No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC4930.10660750274627848No Hit
CCCATGGGGTGTCCTGTCACAGGTACAGCTGCAGCAGTCAGGTCCAGGGC4880.10552629075088012No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA4870.10531004835180043No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC4820.10422883635640207No Hit
GTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGCGAGAGTGAATTCTGTCCCAGATCCACTGCCG4780.10336386676008338No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC4740.1024988971637647No Hit
ATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGA4680.10120144276928666No Hit
CTATAAGACGTCTAGTTTAGAAAATGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCT4650.10055271557204765No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTATTA300.00324783263.61436570
TACTAGA306.3225586E-445.97776431
CGTACTA306.339448E-445.95288529
CTAGATC350.00134946839.4137833
ACCGTAC350.001354505239.38392627
TATAGTA350.001359557339.3541152
GTGTATA451.01183716E-438.26094413
GTACTAG400.002607806634.46466430
TAAAGGT500.003035642233.41151469
ATCAACG9500.033.3474353
TCAACGC9500.033.3474354
ACGCAGA9850.032.5120937
CAACGCA9900.032.347895
GCTTGTA751.6854483E-632.1531031
AACGCAG10050.031.8650866
AGAGTAC10150.031.55114211
CGCAGAG10200.031.3964828
TGTATAA553.290308E-431.30440914
CGTCAGA553.290308E-431.3044095
ACTCTTG450.00428801431.13736360
Produced by FastQC (version 0.11.5)