FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757062

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757062
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2478284
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACAGCCGTGTCAGCGGCGGTCACAGAGCTCAGCTTCAGGGAGAACTGGTTCTTGGATGTGTCTACGGA70610.284914884654059No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT54940.22168565023217676No Hit
GTCTCTGGTGGCTCCATCAGCAGTAGTACTTACTTCTGGGGCTGGATCCG52990.21381730261745627No Hit
GTGTATTACTGTGCGAGACTCCGCTTGACTGGGACCGGCGGAAGGGGTTACTCTGACCACTGGGGCCAGGGAACC46460.18746842573328965No Hit
GTTTTATACAGGTCCGATAATAAGAACTACTTAGGTTGGTACCAGCAGAA45080.18190005665210282No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT43900.17713869758268222No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT43740.17649308957326926No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC43140.17407205953797064No Hit
GAGTAACCCCTTCCGCCGGTCCCAGTCAAGCGGAGTCTCGCACAGTAATA40700.16422653739442292No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC40620.16390373338971642No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC39330.1586985188138244No Hit
GGGTTACTCTGACCACTGGGGCCAGGGAACCCTGATCACCGTCTCCTCAGCCTCCACCAAGGGCCCATCGGTCTT39010.15740730279499848No Hit
ATATTATAGTAGTCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT38630.1558739837726427No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT36760.14832844016262867No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC35410.1428811225832068No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC35370.14271972058085353No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT35180.14195306106967562No Hit
GTATAAAACACTCTGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA34970.14110570055732113No Hit
CACCTGCACTGTCTCTGGTGGCTCCATCAGCAGTAGTACTTACTTCTGGG34520.13928992803084714No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG31900.12871809687670988No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC31780.12823389086965015No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG29420.1187111727308089No Hit
GCTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAACCTAAGTAGTTCTTATTATCGGACCTGTATAAAACACT28440.11475682367315448No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG28290.11415156616432984No Hit
CTATAATATTGTTGACAGTAATAAAGTGCCACATCTTCAGCCTGTAGGCTGTTGATGGTGAGAGTGAAATCTGTC27710.11181123713020784No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT27660.11160948462726628No Hit
CTCCTAAGCTACTCATTTACGGGGCATCTACCCGGGAATCCGGGGTCCCT27380.11047967061079358No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC27140.10951125859667415No Hit
CAGTAATACACAGCCGTGTCAGCGGCGGTCACAGAGCTCAGCTTCAGGGA27120.10943055759549754No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT27000.10894635158843781No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG26790.1080989910760833No Hit
GTAATAAAGTGCCACATCTTCAGCCTGTAGGCTGTTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA26600.10733233156490539No Hit
ATAGTAGTCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGA26280.10604111554607946No Hit
TCTTCAGCCTGTAGGCTGTTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACC26180.10563761054019635No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT25800.10410429151784058No Hit
GTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCGGATTCCCGGGT25400.10249027149430816No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT25330.10220781799018999No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG25240.1018446634848952No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG25000.10087625147077574No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17850.040.119391
GTATCAA37050.037.5424271
GTGGTAT6900.035.926311
TGGTATC7650.031.9469762
TATCAAC43300.031.8778692
ATCAACG44300.031.313683
TCAACGC44600.031.1802334
CAACGCA45700.030.35445
AACGCAG46250.029.9964546
CTACGGA23250.028.72696169
AGAGTAC52600.026.58281511
CGCAGAG53100.026.3314448
GCAGAGT55400.025.3004259
CTACTCA30000.024.62867770
TTCGGGG21650.024.16105870
ACGCAGA58000.023.9828117
AGTACGG51100.023.1171313
GAGTACG51600.022.89312612
GTGTCTA26000.022.3745365
TTAACGG1554.0654413E-822.2713835
Produced by FastQC (version 0.11.5)