FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757063

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757063
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences825861
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT33120.40103600969170355No Hit
GACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACCCGAGTTCCACTCGTTGTATACGAGTTGCAGTAATAAT32390.39219674981625235No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA30130.3648313699278692No Hit
CTCCTGGACAGTCGGTCACCATCTCCTGCACTGGAACCAGCAGTGACGTT27600.3341966747430863No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC27040.3274158726468498No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC26340.3189398700265541No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGGTCACCATCTCCTG23800.2881840890900527No Hit
ACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACCCGAGTTCCACTCG23600.2857623740556825No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC23180.2806767724835051No Hit
CTCCTGCACTGGAACCAGCAGTGACGTTGGTACTTATAACTATGTCTCCTGGTACCAACAACACCCAGGCAAAGC22630.2740170561389871No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG22610.27377488463555005No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG21660.26227173822229166No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACCCGAGTTCCACTCGTTGTATACGAGTTG21580.26130305220854355No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC20850.25246379233309235No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC20520.24846796252638156No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGCAACTCGTAT20420.24725710500919645No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG19330.23405875807187893No Hit
GTATACGAGTTGCAGTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGA19130.23163704303750873No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT18760.2271568702239239No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC18310.22170801139659094No Hit
CTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTC18090.21904412485878375No Hit
GATTATTACTGCAACTCGTATACAACGAGTGGAACTCGGGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGT17480.21165789400395466No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACCCGAGTTCCA16970.2054825206663107No Hit
GCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAG16870.2042716631491256No Hit
GTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGG16370.19821737556320007No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG16180.19591674628054845No Hit
CTCGTATACAACGAGTGGAACTCGGGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGTCAGCCCAAGGCCAA15820.1915576592186821No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT15620.1891359441843119No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT14340.17363696796434266No Hit
ACTTATAACTATGTCTCCTGGTACCAACAACACCCAGGCAAAGCCCCCAAAATCATGATTTTTGATGTCAATAAT14010.16964113815763185No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGGTCACCATCT13860.16782485188185423No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGGTCAC13760.16661399436466912No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC13600.16467662233717295No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG13580.16443445083373595No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG12800.1549897621996922No Hit
GTCGGTCACCATCTCCTGCACTGGAACCAGCAGTGACGTTGGTACTTATAACTATGTCTCCTGGTACCAACAACA12750.15438433344109964No Hit
GAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAGCGATT12490.15123610389641842No Hit
GCCTGGAGCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCC11500.13924861447628595No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA11340.1373112424487898No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT11200.13561604192473067No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGGTCAC11160.13513169891785662No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA11100.13440518440754556No Hit
GGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCC10920.1322256408766124No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG10470.12677678204927947No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC10410.12605026753896842No Hit
TTGTATACGAGTTGCAGTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGGCCGTGTTGC10300.12471832427006482No Hit
GGCCTGGGCTCTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC10170.12314420949772419No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA10110.12241769498741314No Hit
GACCATCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGCAACTCGTATACAACGAGTGGAACTCGGGT10070.12193335198053909No Hit
CAACAACACCCAGGCAAAGCCCCCAAAATCATGATTTTTGATGTCAATAATCGGCCCTCAGGGGTTTCTAATCG9460.11454712112571003No Hit
GGTACCAACAACACCCAGGCAAAGCCCCCAAAATCATGATTTTTGATGTC9450.1144260353739915No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAC9330.11297300635336939No Hit
TTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG9250.11200432033962131No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGC9090.11006694831212516No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT9080.10994586256040666No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT8980.10873500504322156No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC8950.10837174778806603No Hit
CCCCTGAGGGCCGATTATTGACATCAAAAATCATGATTTTGGGGGCTTTGCCTGGGTGTTGTTGGTACCAGGAGA8870.10740306177431796No Hit
CGTTGGTACTTATAACTATGTCTCCTGGTACCAACAACACCCAGGCAAAGCCCCCAAAATCATGATTTTTGATGT8810.1066765472640069No Hit
TCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAGCGATTAGAAACC8710.1054656897468218No Hit
GGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAGCGATTAGAAA8700.10534460399510329No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG8700.10534460399510329No Hit
GGCCTGGAAGGCAGATGGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA8690.10522351824338479No Hit
GTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTT8630.10449700373307373No Hit
CTTATAACTATGTCTCCTGGTACCAACAACACCCAGGCAAAGCCCCCAAA8620.1043759179813552No Hit
CTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCG8430.10207528869870354No Hit
GATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGG8310.10062225967808142No Hit
CGTATACAACGAGTGGAACTCGGGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGTCAGCCCAAGGCCAACC8260.10001683091948887No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAT150.002333539869.1617439
ATCGCGT200.007327421651.80505437
CGTTACC200.007460473651.5698217
GTATCAA18700.039.384361
GGTATCA7900.038.771671
CGAGACG1350.035.65968723
GTGGTAT2800.035.644421
TATCAAC20950.035.446562
CGTATGC709.450978E-734.62937541
TCGTATG501.82635E-434.6103840
AGACGTA1400.034.39029325
CGAGCCC1300.034.3819615
CGTAGAG1450.033.2245428
GACGTAG1450.033.21045326
TCAACGC22400.033.1520274
ATCAACG22450.032.9250533
TATGCCG751.5996866E-632.37405843
CAACGCA23150.032.2264985
AACGCAG23600.031.612016
ACGAGAC1550.031.05655722
Produced by FastQC (version 0.11.5)