FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757064

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757064
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences825861
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT30870.37379171555503893No Hit
GACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACCCGAGTTCCACTCGTTGTATACGAGTTGCAGTAATAAT28690.34739502168040387No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC27550.33359124598449374No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA27130.3285056444123164No Hit
CTCCTGGACAGTCGGTCACCATCTCCTGCACTGGAACCAGCAGTGACGTT26720.32354112859185746No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGGTCACCATCTCCTG25850.3130066681923471No Hit
CTCCTGCACTGGAACCAGCAGTGACGTTGGTACTTATAACTATGTCTCCTGGTACCAACAACACCCAGGCAAAGC24250.2936329479173856No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC23880.2891527751038008No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG21520.2605765376982325No Hit
ACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACCCGAGTTCCACTCG21380.2588813371741734No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC20800.2518583635744998No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC20700.25064750605731473No Hit
CTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTC20340.24628841899544837No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG19820.2399919599060859No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG19200.23248464329953827No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGCAACTCGTAT19080.23103161427891616No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC19040.23054727127204216No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACCCGAGTTCCACTCGTTGTATACGAGTTG18840.22812555623767197No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT18260.22110258263799842No Hit
GTATACGAGTTGCAGTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGA17660.21383743753488785No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC17210.2083885787075549No Hit
GATTATTACTGCAACTCGTATACAACGAGTGGAACTCGGGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGT16540.20027583334241475No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16160.1956745747771114No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG16110.19506914601851885No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACCCGAGTTCCA15670.18974137294290444No Hit
CTCGTATACAACGAGTGGAACTCGGGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGTCAGCCCAAGGCCAA15420.1867142291499417No Hit
GCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAG15370.18610880039134917No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGGTCAC15280.18501902862588257No Hit
GTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGG15120.1830816565983864No Hit
ACTTATAACTATGTCTCCTGGTACCAACAACACCCAGGCAAAGCCCCCAAAATCATGATTTTTGATGTCAATAAT14460.17508999698496477No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT14170.171578510185128No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG13830.16746159462669868No Hit
GTCGGTCACCATCTCCTGCACTGGAACCAGCAGTGACGTTGGTACTTATAACTATGTCTCCTGGTACCAACAACA13290.16092296403389916No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGGTCACCATCT13190.15971210651671408No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT13080.15838016324781048No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGGTCAC12620.15281021866875902No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC12420.15038850363438883No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA12130.14687701683455207No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT11820.14312335853127828No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG11240.1361003849316047No Hit
GCCTGGAGCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCC11180.13537387042129367No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG11080.13416301290410856No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT10850.13137804061458283No Hit
GGCCTGGGCTCTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC10610.1284719825733386No Hit
GAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAGCGATT10440.12641352479412396No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA10390.12580809603553142No Hit
GACCATCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGCAACTCGTATACAACGAGTGGAACTCGGGT10210.12362855250459823No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC9850.1192694654427319No Hit
GGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCC9830.11902729393929487No Hit
TTGTATACGAGTTGCAGTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGGCCGTGTTGC9720.11769535067039126No Hit
TCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCT9650.11684775040836169No Hit
TTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG9400.11382060661539894No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGC9310.11273083484993239No Hit
GGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAGCGATTAGAAA9300.11260974909821386No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA9280.11236757759477685No Hit
CAACAACACCCAGGCAAAGCCCCCAAAATCATGATTTTTGATGTCAATAATCGGCCCTCAGGGGTTTCTAATCG9090.11006694831212516No Hit
GCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGGTCACCATCTCCTGCACTGGAACCAGCAGTGACGTTGGTA8930.10812957628462902No Hit
GGCTGAGGACGAGGCTGATTATTACTGCAACTCGTATACAACGAGTGGAA8850.10716089027088094No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC8810.1066765472640069No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT8770.10619220425713286No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGG8720.1055867754985403No Hit
GTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTT8630.10449700373307373No Hit
CTTATAACTATGTCTCCTGGTACCAACAACACCCAGGCAAAGCCCCCAAA8600.1041337464779182No Hit
AGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCG8570.10377048922276266No Hit
GGCCTGGAAGGCAGATGGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA8480.1026807174572961No Hit
ACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC8480.1026807174572961No Hit
CCCCTGAGGGCCGATTATTGACATCAAAAATCATGATTTTGGGGGCTTTGCCTGGGTGTTGTTGGTACCAGGAGA8440.10219637445042205No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAC8380.10146985994011097No Hit
GGTACCAACAACACCCAGGCAAAGCCCCCAAAATCATGATTTTTGATGTC8320.10074334542979993No Hit
CTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGAT8300.10050117392636292No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8000.043.3800241
CGATTGT608.49692E-443.2992670
TGGTATC2400.041.5087782
GTGGTAT2450.040.671511
GTATCAA18700.038.0353241
TATCAAC20350.034.60532
TCAACGC21000.033.6977654
ATCAACG20950.033.6142163
TAGCGGC859.882933E-833.22036764
CAACGCA21350.033.1453445
AACGCAG22100.032.028266
CGCAGAG23200.030.3726088
AGAGTAC24000.029.64842611
ACGCAGA23850.029.5430557
AGTACGG16700.029.43486413
TACGGGG11000.029.37320715
GAGTACG16900.029.08652312
CAGAGTA24500.028.90129910
GCAGAGT24550.028.8424349
ACGGGGG5900.028.54352816
Produced by FastQC (version 0.11.5)