FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005757065

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757065
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences442349
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT19090.43155969607707945No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC13830.3126490621658464No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT12670.28642542426907264No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG12600.28484296336150866No Hit
GGTCTACTCAGAGCCTCCTGCATAGTAATGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTC12360.27941738310700376No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12240.2767045929797513No Hit
GTGCAGCCACAGTTCGTATGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG11850.2678880250661808No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG11680.2640449057192398No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC11510.2602017863722988No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT11450.25884539130867257No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC10630.24030799210578072No Hit
GTCCCTGACAGGTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAA10540.23827339951034138No Hit
GTAATAGACGGCCGTGTCCGCAGCGGTCACAGAGTTCAACTTCAGGGAGAACTGGTTCTGGGACGCGTCTACTGA10360.2342042143194627No Hit
GTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAATCTGTGCCTGATCCACTGCCA9720.21973600030744952No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT9600.21702321018019707No Hit
AGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGGGTTCTAATCGGGCC9550.21589288096050857No Hit
GTAATGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGGGTT9330.21091943239387903No Hit
GTCAGGGACCCCGGAGGCCCGATTAGAACCCAAATAGATCAGGAGCTGTGGAGACTGCCCTGGCTTCTGCAGGTA9200.20798057642268888No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCATACGAACTGTGGCTGCACCA8890.20097253526062003No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG8510.1923820331909872No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG8240.18627825540466916No Hit
CCCCTGGAGAGTCGGCCTCCATCTCCTGCAGGTCTACTCAGAGCCTCCTG8060.18220907021379046No Hit
GGCCAAAAGTGTACGGAATTTGTAGACCTTGCATGCAGTAATAAACCCCA8030.18153087268197735No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC7980.18040054346228881No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT7820.17678348995928553No Hit
GTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAAAATCAGCAGAG7740.17497496320778388No Hit
CCCCTGGAGAGTCGGCCTCCATCTCCTGCAGGTCTACTCAGAGCCTCCTGCATAGTAATGGATACAACTATTTGG7740.17497496320778388No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG7590.17158397554871832No Hit
GTCTACAAATTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCATA7490.16932331710934126No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTATGATCTCCAGCTTGGTCC7450.16841905373359045No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC7080.16005461750789535No Hit
CTCCAGGGGTGACGGGCAGGGAGAGTGGAGACTGAGTCATCACAATATCC7020.15869822244426912No Hit
CAGTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAATCTGTGCCTGATCCACTGCCA6850.1548551030973281No Hit
GTCTCCACAGCTCCTGATCTATTTGGGTTCTAATCGGGCCTCCGGGGTCC6760.15282051050188877No Hit
GTCTATTACTGTGCGAGAGGGATGATCACTTTGTTTCGGGGGGGACCAGT6640.1501077203746363No Hit
ACTCAGAGCCTCCTGCATAGTAATGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAG6590.1489773911549478No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG6450.14581246933981992No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCACTCTCC6410.14490820596406911No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG6360.14377787674438058No Hit
GTCTCCACTCTCCCTGCCCGTCACCCCTGGAGAGTCGGCCTCCATCTCCT6340.14332574505650514No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA6330.14309967921256744No Hit
GAGTAGACCTGCAGGAGATGGAGGCCGACTCTCCAGGGGTGACGGGCAGG6280.1419693499928789No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCACT6250.1412911524610658No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT6210.140386889085315No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA6170.13948262570956416No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGA6140.13880442817775104No Hit
GCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCACTCTCCCTGCCCGTCACCC6130.13857836233381335No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA6120.13835229648987565No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA6070.13722196727018712No Hit
GTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGGGTTCTAATCGGGCCTCCGGGGTCCCTGA6070.13722196727018712No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG5990.13541344051868548No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG5990.13541344051868548No Hit
CACCTGCACTGTCTCTCGTGGCTCCATCAATCATTACTACTGGAGCTGGTTCCGGCAGCCCCCAGGGAAGGGA5990.13541344051868548No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT5870.132700650391433No Hit
TTATTACTGCATGCAAGGTCTACAAATTCCGTACACTTTTGGCCAGGGGA5850.1322485187035576No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT5810.13134425532780677No Hit
GTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAAGGTCTACAAAT5800.13111818948386908No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT5730.12953572857630513No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG5710.1290835968884297No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG5560.12569260922936415No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT5550.12546654338542645No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA5520.12478834585361333No Hit
GCCCTGGCTTCTGCAGGTACCAATCCAAATAGTTGTATCCATTACTATGC5470.12365801663392481No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA5450.12320588494604938No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC5410.12230162157029856No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT5390.12184948988242315No Hit
GAGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAAGGTCTACAAATTCCGTACACTTTTGGCCAGGGGA5230.11823243637941988No Hit
CTCCACAGCTCCTGATCTATTTGGGTTCTAATCGGGCCTCCGGGGTCCCT5210.11778030469154446No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC5180.11710210715973135No Hit
GATTTTACACTGAAAATCAGCAGAGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAAGGTCTACAAATT5070.11461538287641658No Hit
CTCCTGCATAGTAATGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGAT5050.11416325118854118No Hit
GCATAGTAATGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGC5030.11371111950066576No Hit
GAATTTGTAGACCTTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAAT5030.11371111950066576No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT5010.11325898781279035No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT4950.11190259274916413No Hit
ATGCAGGAGGCTCTGAGTAGACCTGCAGGAGATGGAGGCCGACTCTCCAGGGGTGACGGGCAGGGAGAGTGGAGA4880.1103201318416002No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG4870.11009406599766247No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG4840.10941586846584936No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC4830.10918980262191165No Hit
CCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCT4830.10918980262191165No Hit
GTTTATTACTGCATGCAAGGTCTACAAATTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCATACGAA4820.10896373677797394No Hit
GCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGGGTTCTAATCGGGCCTCCGGGGTCCCTGACAGGTT4800.10851160509009854No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA4700.10625094665072149No Hit
GTTCGTATGATCTCCAGCTTGGTCCCCTGGCCAAAAGTGTACGGAATTTGTAGACCTTGCATGCAGTAATAAACC4680.10579881496284609No Hit
ATCCATTACTATGCAGGAGGCTCTGAGTAGACCTGCAGGAGATGGAGGCC4640.10489455158709526No Hit
TTTGTAGACCTTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAAT4620.10444241989921986No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC4600.10399028821134444No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC4480.10127749808409196No Hit
GTGGAGACTGCCCTGGCTTCTGCAGGTACCAATCCAAATAGTTGTATCCA4450.10059930055227885No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)