FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757066

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757066
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences442349
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT18300.4137004944060007No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT13010.2941116629629546No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC12900.29162493867963984No Hit
GGTCTACTCAGAGCCTCCTGCATAGTAATGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTC12280.2776088563555021No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG11930.26969655181768243No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT11660.2635927740313644No Hit
GTGCAGCCACAGTTCGTATGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG11180.2527416135223545No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC10890.24618570404816106No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC10050.22719617315739385No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9670.21860567108776102No Hit
GTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAATCTGTGCCTGATCCACTGCCA9650.21815353939988563No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT9540.21566681511657085No Hit
GTAATAGACGGCCGTGTCCGCAGCGGTCACAGAGTTCAACTTCAGGGAGAACTGGTTCTGGGACGCGTCTACTGA9470.21408435420900693No Hit
GTCCCTGACAGGTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAA9430.21318009083325606No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT9010.20368532538787248No Hit
GGCCAAAAGTGTACGGAATTTGTAGACCTTGCATGCAGTAATAAACCCCA8850.20006827188486917No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC8540.19306023072280032No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCATACGAACTGTGGCTGCACCA8110.183339399433479No Hit
AGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGGGTTCTAATCGGGCC7990.18062660930622654No Hit
GTCAGGGACCCCGGAGGCCCGATTAGAACCCAAATAGATCAGGAGCTGTGGAGACTGCCCTGGCTTCTGCAGGTA7810.17655742411534783No Hit
CCCCTGGAGAGTCGGCCTCCATCTCCTGCAGGTCTACTCAGAGCCTCCTGCATAGTAATGGATACAACTATTTGG7730.1747488973638462No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG7490.16932331710934126No Hit
GTAATGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGGGTT7310.16525413191846258No Hit
GTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAAAATCAGCAGAG7180.1623152759472724No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT7070.15982855166395765No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG7030.15892428828820682No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG6980.1577939590685183No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTATGATCTCCAGCTTGGTCC6970.1575678932245806No Hit
GTCTACAAATTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCATA6710.15169018128220027No Hit
CAGTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAATCTGTGCCTGATCCACTGCCA6640.1501077203746363No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA6630.1498816545306986No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT6600.1492034569988855No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA6590.1489773911549478No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT6570.14852525946707237No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT6470.14626460102769534No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG6300.14242148168075433No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG6270.14174328414894122No Hit
CACCTGCACTGTCTCTCGTGGCTCCATCAATCATTACTACTGGAGCTGGTTCCGGCAGCCCCCAGGGAAGGGA6270.14174328414894122No Hit
CTCCAGGGGTGACGGGCAGGGAGAGTGGAGACTGAGTCATCACAATATCC6270.14174328414894122No Hit
GTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAAGGTCTACAAAT6220.1406129549292527No Hit
GTCTCCACTCTCCCTGCCCGTCACCCCTGGAGAGTCGGCCTCCATCTCCT6200.1401608232413773No Hit
GTCTATTACTGTGCGAGAGGGATGATCACTTTGTTTCGGGGGGGACCAGT6150.13903049402168877No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT6130.13857836233381335No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGA6110.13812623064593793No Hit
CCCCTGGAGAGTCGGCCTCCATCTCCTGCAGGTCTACTCAGAGCCTCCTG6100.13790016480200024No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA6100.13790016480200024No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA6080.13744803311412482No Hit
ACTCAGAGCCTCCTGCATAGTAATGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAG6030.1363177038944363No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG6030.1363177038944363No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCACT6020.1360916380504986No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA6020.1360916380504986No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG5880.13292671623537072No Hit
GTCTCCACAGCTCCTGATCTATTTGGGTTCTAATCGGGCCTCCGGGGTCC5880.13292671623537072No Hit
GTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGGGTTCTAATCGGGCCTCCGGGGTCCCTGA5820.1315703211717445No Hit
GAGTAGACCTGCAGGAGATGGAGGCCGACTCTCCAGGGGTGACGGGCAGG5780.13066605779599366No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA5730.12953572857630513No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG5680.1284053993566166No Hit
GCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCACTCTCCCTGCCCGTCACCC5630.12727507013692807No Hit
TTATTACTGCATGCAAGGTCTACAAATTCCGTACACTTTTGGCCAGGGGA5580.12614474091723957No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCACTCTCC5580.12614474091723957No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG5560.12569260922936415No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA5500.12433621416573792No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC5280.1193627655991084No Hit
GAATTTGTAGACCTTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAAT5260.118910633911233No Hit
CTCCACAGCTCCTGATCTATTTGGGTTCTAATCGGGCCTCCGGGGTCCCT5100.11529358040822969No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC5010.11325898781279035No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC4960.11212865859310184No Hit
GAGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAAGGTCTACAAATTCCGTACACTTTTGGCCAGGGGA4940.11167652690522642No Hit
CTCCTGCATAGTAATGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGAT4910.11099832937341331No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC4870.11009406599766247No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT4800.10851160509009854No Hit
GTGTAAAATCTGTGCCTGATCCACTGCCACTGAACCTGTCAGGGACCCCG4710.1064770124946592No Hit
GTTTATTACTGCATGCAAGGTCTACAAATTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCATACGAA4610.10421635405528215No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT4520.10218176145984278No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA4500.10172962977196738No Hit
GATTTTACACTGAAAATCAGCAGAGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAAGGTCTACAAATT4460.10082536639621656No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC4440.10037323470834116No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGTTT451.7164095E-459.86379670
CCGTTAA200.005360746356.05900668
GTGGTAT900.053.6127551
TGGTATC950.050.779552
GGTATCA3050.041.8104171
AGAGCAT2900.039.47914570
GTATCAA7100.037.3779071
GGGATAC807.133167E-834.46924218
TTCATAC601.3520377E-534.457553
TATCAAC7800.033.574022
ATCAACG8000.032.7346733
TCAACGC8150.032.1321954
CAACGCA8450.030.9914075
AACGCAG8600.030.4543026
CGTCTAC2800.030.19380666
CGGGATA802.7758142E-630.16058517
CTACTGA3400.029.64876269
CAGAGTA8900.029.05039610
GGATACT953.2149546E-729.03329519
CGCGTCT2900.028.97516864
Produced by FastQC (version 0.11.5)