FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757070

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757070
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1025417
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT29350.28622501869970945No Hit
CTCTACAACCTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAG24320.2371718042513436No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT23890.2329783883044654No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT21640.2110360955591725No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT20060.19562772998692238No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC19060.18587559987790334No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC18620.18158466262993495No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT18420.17963423660813113No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC18040.1759284271667039No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC17960.17514825675798235No Hit
GTGCAGCCACAGTTCGCTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG17700.1726127029296374No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG16610.16198288111080664No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT16410.16003245508900282No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16220.1581795503682892No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACACCTGTGGTTCTTCCTCCTGCTGGTGGCAGCTCCCAGATGGGTC15900.1550588687334031No Hit
GTCCCTGACAGGTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAA15750.15359604921705022No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG15740.15349852791596003No Hit
GGCCTGGAGTGGATTGGGTACATCTATTACACTGGGAATACCTTCTACAA15410.15028032497998375No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC14540.14179597178513717No Hit
GTTGTAGAGACTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAAT14200.1384802475480707No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT14120.13770007713934915No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT14090.13740751323607858No Hit
AGCCAGGGCAGTCTCCACAGCTCCTGATCTTTTTGAGTTCTAATCGGGCC13670.1333116185902906No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC13540.1320438416761181No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAGCGAACTGTGGCTGCACCA13530.1319463203750279No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC13340.1300934156543143No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG13230.1290206813423222No Hit
GGTTGTAGAGACTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAAT13100.1277529044281497No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA13090.1276553831270595No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC13080.12755786182596934No Hit
GGTCTAGTCAGAGCCTCCTGCATAGTAATGGATATAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTC13010.12687521271833802No Hit
GTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAATCTGTGCCTGATCCACTGCCA12440.12131649855619712No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT12230.11926855123330314No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT12070.11770821041586009No Hit
GTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAAAATCAGCAGAG11820.11527017788860532No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG11820.11527017788860532No Hit
GTGTCTGGTTTCTCCATCATCAGTGGTGGTTACGACTGGAGCTGGATCCG11750.11458752878097397No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT11680.11390487967334266No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG11660.11370983707116226No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT11610.11322223056571132No Hit
CCCCTGGAGCGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTG11570.11283214536135056No Hit
GGCCAAAAGTGTACGGAGGTTGTAGAGACTGCATGCAGTAATAAACCCCA11280.11000402762973503No Hit
GTAATGGATATAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTTTTTGAGTT11270.10990650632864483No Hit
GTCTCCACAGCTCCTGATCTTTTTGAGTTCTAATCGGGCCTCCGGGGTCC11220.10941889982319387No Hit
CTCCAGGCCCGTCCCTGGGGTCTGGCGGATCCAGCTCCAGTCGTAACCAC11170.10893129331774293No Hit
GGGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTT11160.10883377201665273No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA10960.10688334599484892No Hit
GTCTCCACTCTCCCTGCCCGTCACCCCTGGAGCGCCGGCCTCCATCTCCT10730.10464035606977454No Hit
CTATTACACTGGGAATACCTTCTACAACCCGTCCCTCAAGAGTCGAGTCACCATATCAAGAGACCCGTCTAAGAA10600.10337257915560207No Hit
GTCAGGGACCCCGGAGGCCCGATTAGAACTCAAAAAGATCAGGAGCTGTGGAGACTGCCCTGGCTTCTGCAGGTA10590.10327505785451188No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC10540.10278745134906092No Hit
GTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT10530.10268993004797072No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG10270.10015437621962577No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATTC200.00737890951.7143063
GGTATCA7100.040.3129961
GTATCAA14700.037.7688641
GTGGTAT2600.033.158281
TATCAAC17250.031.9779262
ATCAACG17750.031.2713743
TGGTATC2800.030.7823242
TCAACGC17700.030.7753684
TAGTACC450.00463808930.6455144
AACGCAG18050.030.1815596
CAACGCA18200.029.929895
AGAGTAC19600.027.82324611
CGCAGAG20050.027.370938
GGGAACG4300.027.10094370
CAGAGTA20400.026.90132910
ATTAGAG658.691402E-426.5201573
ACGCAGA21700.025.2835587
TACACCC8600.023.6522795
GAGTACT4700.023.49951612
GCATAGG902.1325704E-422.9897421
Produced by FastQC (version 0.11.5)