FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757073

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757073
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1076671
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA31000.043.8518451
GGTATCA11850.040.4715421
TATCAAC37400.036.6809922
ATCAACG37500.036.21553
TCAACGC37450.036.1718184
CAACGCA38300.035.459045
AACGCAG39100.034.733546
ACGCAGA40450.033.5743267
GTGGTAT4500.032.9692651
AGAGTAC41850.032.6174111
CGCAGAG42350.032.0695278
CAGAGTA43100.031.59145210
GCAGAGT44300.030.657899
TGGTATC4850.029.1388032
GAGTACG35700.028.00138512
AGTACGG35700.028.00138513
TAGCGGC2850.027.1370164
ACGGGTA1457.1122486E-1026.1489516
GTACGGG39650.024.77715314
AATAGCG3350.024.08135662
Produced by FastQC (version 0.11.5)