FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757074

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757074
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1076671
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15180.14099014462170897No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14450.043.8146441
GTATCAA33300.039.265281
GTGGTAT5650.035.3222921
TGGTATC5600.035.011842
TATCAAC39350.032.6056022
TCAACGC40000.031.9897674
ATCAACG40800.031.615443
CAACGCA41200.031.3084935
AACGCAG42950.030.2744986
ACGCAGA44000.029.4093347
AGAGTAC45900.028.4287811
CAGAGTA46050.028.41094210
CGCAGAG45750.028.3714778
GCAGAGT48050.027.300049
CGAGACG1407.448021E-526.83992470
AGTACGG38750.026.83285713
GAGTACG38900.026.72938712
TACGGGT5900.025.0869115
GTACGGG42650.024.2166314
TACGGGA13000.023.56554215
Produced by FastQC (version 0.11.5)