FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757075

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757075
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1518063
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA31260.2059203076552159No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC31000.20420759876237018No Hit
GCCTTGGGCTGACCGAGGACGGCGACCTTGGTCCCAGTTCCGAAGACGTGACCATTCAGGCTGTCATCCCATG28400.18708050983391333No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT27540.18141539580373145No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC26640.17548678809772716No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT25690.16922881329694486No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG22760.1499279015429531No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC22390.1474905850415958No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT21550.1419572178493251No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT21160.13938815451005657No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT21060.13872942032050053No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC20890.13760957219825526No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT20820.13714845826556604No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC20540.13530400253480918No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC20170.13286668603345184No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC19800.13042936953209452No Hit
GTAATAATCACCCTCATCCTCAGACTGGAGCCCACTGATGGCCAGGGAGC19790.1303634961131389No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG18540.1221293187436885No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC18470.12166820481099927No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG18150.11956025540441996No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC17550.11560785026708377No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCGAGGACGGCGACCTTGGTC17380.11448800214483852No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA16390.10796653366823379No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG16380.1079006602492782No Hit
CCCTAAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTAGTCGCTTTGCC15700.10342126776029717No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC15560.10249903989491872No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT15530.10230141963805191No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT15450.10177443228640709No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCGT200.007247851.95052742
TCGTATG506.7901055E-848.4406840
GTGGTAT3950.047.189641
GTATCAA29800.042.1633571
GGTATCA12150.040.058331
TGGTATC4850.036.2593882
TATCAAC34750.036.218472
ATCAACG36000.034.86513
CAACGCA36300.034.5769585
TCAACGC36300.034.4819684
AACGCAG36850.033.967316
AGAGTAC38500.032.51478211
CGTATGC751.627579E-632.30553441
CGCAGAG39400.031.7710118
CAGAGTA40100.031.30343210
TCTCGTA901.9597974E-730.73059738
TATCTCG901.9683284E-730.71538536
GCAGAGT41600.030.2566039
AGTACGG34150.028.77988613
GAGTACG34750.028.18373112
Produced by FastQC (version 0.11.5)