FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757077

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757077
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences730441
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT8430.1154097319290675No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA18650.042.5346641
TATCAAC21250.037.135042
TCAACGC21350.036.63834
CAACGCA21750.036.122935
ATCAACG21900.035.7181633
AACGCAG22600.034.9167986
GGTATCA7650.034.264641
ACGCAGA23900.033.161747
CGCAGAG24200.032.750648
AGAGTAC24500.032.21337511
CAGAGTA26050.030.56124910
GCAGAGT26550.029.8538539
GTGGTAT2600.029.18391
ACCGATC953.2226671E-729.03034427
GAGTACG19700.027.81619312
AGTACGG19700.027.81619313
AGTATCG500.00769747427.62612534
TCTACGC1705.456968E-1226.3512842
TACGGGG8450.025.6951215
TGGTATC3400.025.3377722
Produced by FastQC (version 0.11.5)