FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757078

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757078
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences730441
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10680.14621304116280437No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT8640.11828470745754963No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6900.045.864851
GTATCAA20900.043.7800831
TATCAAC23500.038.784642
TCAACGC24200.037.3785254
ATCAACG24700.036.761123
CAACGCA24750.036.5478945
AACGCAG25050.036.3897746
AATCGGA501.890923E-434.40570417
ACGCAGA26900.034.16167
AGAGTAC26750.033.9741511
CGCAGAG27400.033.9196938
CAGAGTA28450.032.4280610
TACGAAT800.003801305831.91225270
GCAGAGT29050.031.8767919
AGTACGG23650.029.69391613
GAGTACG24300.028.89963312
TACGGGC4550.028.75018515
TAGTTAG900.006761554628.36644770
TAATACC854.532556E-628.3244114
CGACCGT500.007743234327.59258532
Produced by FastQC (version 0.11.5)