FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757080

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757080
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1013174
Sequences flagged as poor quality0
Sequence length20-76
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC57270.5652533523363213No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG56300.5556794785495877No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT52700.5201475758359373No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC42260.41710505796635133No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC39350.3883834366061506No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA36320.3584774184888282No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC36050.3558125257853044No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC34680.3422906628081652No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG34500.3405140676724827No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG33850.3340985852380736No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT31560.31149634712300156No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG29630.2924472992792945No Hit
GCCTTGGGCTGACCGAGGACGGCGACCTTGGTCCCAGTTCCGAAGACGTGACCATTCAGGCTGTCATCCCATG27910.27547094576055053No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG25360.2503025146717148No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA24100.2378663487219372No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG23330.23026646953040641No Hit
GATTATTACTGTTCAGCATGGGATGACAGCCTGAATGGTCACGTCTTCGG23140.22839117466496375No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT22490.2219756922305547No Hit
GTCTGAGGATGAGGGTGATTATTACTGTTCAGCATGGGATGACAGCCTGAATGGTCACGTCTTCGGAACTGGGAC22360.22069259574367286No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT22300.22010039736511203No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT22170.2188173008782302No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAG20960.20687463357725328No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT20780.20509803844157076No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC20700.20430844060348963No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT20360.20095264979164487No Hit
GAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAG19560.19305667141083369No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG19530.19276057222155327No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC19410.19157617546443156No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAC19400.19147747573467144No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC19270.19019437924778962No Hit
ACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC19080.18831908438234696No Hit
GGATGACAGCCTGAATGGTCACGTCTTCGGAACTGGGACCAAGGTCGCCGTCCTCGGTCAGCCCAAGGCCAACCC19050.18802298519306654No Hit
GGCCTGGAAGGCAGATGGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA18590.1834827976241001No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT18560.1831866984348197No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT18480.18239710059673855No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGGCTCCCTGGCCATCAGTGGGCTCCAGT18310.1807192051908162No Hit
GTATTACTTCCGATGTTGGAACTGCTTCCAGAACAAGAGATGGTGACCCT17950.17716601491945116No Hit
GGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCC17790.17558681924328892No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA17610.17381022410760638No Hit
CCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCT17350.17124403113384273No Hit
GCATGGGATGACAGCCTGAATGGTCACGTCTTCGGAACTGGGACCAAGGT17320.17094793194456234No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCGAGGACGGCGACCTTGGTC17240.17015833410648123No Hit
GTTCAGCATGGGATGACAGCCTGAATGGTCACGTCTTCGGAACTGGGACC17100.16877653788983926No Hit
CTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGAT17100.16877653788983926No Hit
CTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCG16710.1649272484291938No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCTATAGCAAGCTCACCGTGGACAAGAG15770.15564947383174063No Hit
GTCTGGCACCTCAGGCTCCCTGGCCATCAGTGGGCTCCAGTCTGAGGATG15250.15051708788421336No Hit
TTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG15100.14903659193781127No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGG15050.14854309328901058No Hit
TTACTGTTCAGCATGGGATGACAGCCTGAATGGTCACGTCTTCGGAACTG15020.14824699409973016No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC14760.1456808011259665No Hit
GGCCATCAGTGGGCTCCAGTCTGAGGATGAGGGTGATTATTACTGTTCAGCATGGGATGACAGCCTGAATGGTCA14730.1453847019366861No Hit
GGAGTGGAGACCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGC14690.14498990301764555No Hit
CTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCGTCAAGGCGGGAGTG14640.14449640436884484No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT14630.14439770463908472No Hit
GGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCA14530.1434107073414833No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG14530.1434107073414833No Hit
GGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGC14460.14271980923316233No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA14350.14163411220580077No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG14280.14094321409747979No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA13910.13729132409635464No Hit
TGGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAG13760.13581082814995254No Hit
GCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCA13710.13531732950115183No Hit
TTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCA13710.13531732950115183No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT13610.13433033220355042No Hit
GGGCAGTGGTGGGTGCTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTA13580.13403423301427No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGG13530.13354073436546932No Hit
GTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTT13280.1310732411214658No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC13190.13018494355362453No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC13180.1300862438238644No Hit
GAACAAGAGATGGTGACCCTCTGCCCGGGGGTCCCAGACGCTGAGGGTGG13120.12949404544530357No Hit
GCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCA13060.12890184706674274No Hit
CCTCAGGCTCCCTGGCCATCAGTGGGCTCCAGTCTGAGGATGAGGGTGAT12950.1278161500393812No Hit
CCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTG12720.12554605625489798No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG12520.12357206165969517No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC12330.12169676679425251No Hit
CTTCGGAACTGGGACCAAGGTCGCCGTCCTCGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC12110.11952537273952944No Hit
TGGGATGACAGCCTGAATGGTCACGTCTTCGGAACTGGGACCAAGGTCGCCGTCCTCGGTCAGCCCAAGGCCAAC12060.11903187409072874No Hit
GCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAG11990.11834097598240777No Hit
GCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGA11730.11577478300864412No Hit
GGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAAC11730.11577478300864412No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC11710.11557738354912384No Hit
CCCCTGAGGGCCGCTGACTATTAGTAAAGATGAGGAGTTTGGGGGCCGTT11660.11508388490032313No Hit
GAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTC11520.11370208868368117No Hit
GAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG11510.11360338895392104No Hit
GGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGG11500.11350468922416089No Hit
CAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGG11440.11291249084560005No Hit
GGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG11440.11291249084560005No Hit
GTTTGGGGGCCGTTCCTGGGAGTTGCTGGTACCAGTTTATAGTATTACTT11430.11281379111583992No Hit
ATGTTGGAACTGCTTCCAGAACAAGAGATGGTGACCCTCTGCCCGGGGGT11400.11251769192655951No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGG11390.11241899219679938No Hit
TACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTT11380.11232029246703923No Hit
CTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTC11310.11162939435871826No Hit
GATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGG11280.11133329516943784No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCT11200.11054369733135672No Hit
ACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC11200.11054369733135672No Hit
GGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCCGGGGAGCGGGGGCTTGCCGGCCGTCGCACTCATTTA11170.1102475981420763No Hit
GTCCTCGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC10930.10787880462783293No Hit
GGGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTT10900.1075827054385525No Hit
CCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACA10890.10748400570879237No Hit
GTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT10840.10699050705999166No Hit
AGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCATAGGTTCCCAACTCTAACCCCACCCACGGGAG10840.10699050705999166No Hit
GACACACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGG10810.10669440787071124No Hit
AGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGT10600.1046217135457483No Hit
GCCTGAATGGTCACGTCTTCGGAACTGGGACCAAGGTCGCCGTCCTCGGTCAGCCCAAGGCCAACCCCACTGTCA10590.10452301381598816No Hit
CTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTC10570.10432561435646791No Hit
CTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGC10470.1033386170588665No Hit
AGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGA10390.10254901922078538No Hit
GGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGT10270.10136462246366369No Hit
GTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGTGACTT10210.10077242408510285No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTAA250.001140317882.8341370
CCGTTTA200.002933963465.2801569
GTGGTAT1900.052.112711
AGCGTAT200.00766189451.22432310
GGTATCA6750.046.5353851
TGGTATC2300.044.523112
GTATCAA17450.041.871411
TCTGTCG504.1230523E-640.9774368
TGTATAA603.1638046E-739.823452
ACCGTTT606.1752216E-638.63618568
TATCAAC19450.037.7320982
ATCAACG21150.034.537863
TCGTCGA400.002725503934.15966825
ACAGCGT601.4400848E-534.1478658
GTGTATA701.0560034E-634.1428071
ATTGAGT400.00273541834.134386
TCAACGC21500.033.9756164
CAACGCA21700.033.662485
AACGCAG22250.032.9837846
TCATCCA6200.031.17413570
Produced by FastQC (version 0.11.5)