FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757081

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757081
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1693098
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17200.043.477241
GTATCAA42150.039.816411
ATCAACG49100.034.0861173
TCAACGC49050.034.0506554
TATCAAC49400.033.8094022
CAACGCA49600.033.603655
AACGCAG50850.032.7098776
ACGCAGA53950.030.8285277
TACGTAC450.00466360930.61229524
AGAGTAC56800.029.34403211
CGCAGAG57150.029.1032038
CAGAGTA57650.028.91137710
GTGGTAT6900.027.9685291
GCAGAGT60750.027.4352559
TGGTATC6850.026.644532
AGTACGG48800.026.03929913
GAGTACG51400.024.78913312
TTCGCAT851.4617668E-424.30832921
GTACGGG56950.022.25239814
TTAACGG1756.15546E-921.70110735
Produced by FastQC (version 0.11.5)