FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757082

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757082
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1693098
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17830.10530991118056958No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19050.039.3481521
GTATCAA40200.035.838581
GTGGTAT7650.032.3618931
TATCAAC46900.030.6409442
ATCAACG47250.030.2684523
TCAACGC47850.029.888914
CAACGCA48800.029.3775085
AACGCAG50400.028.5156296
TGGTATC8700.027.2664452
ACGCAGA53800.026.7237857
CAGAGTA54550.026.36025810
AGAGTAC54450.026.28231411
CGCAGAG55450.025.9316438
GTCGTAT809.784953E-525.7883831
GCAGAGT57900.024.7756829
CGATTGT2601.4040779E-824.6145870
AGTACGG48850.021.90100113
TAACGGC3050.021.51071536
GAGTACG50300.021.26965912
AACGTTA1550.003730727120.64448770
Produced by FastQC (version 0.11.5)