FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757088

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757088
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences227127
Sequences flagged as poor quality0
Sequence length20-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3270.14397231504840904No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGCAT200.006583959353.21266663
GTATCAA10000.037.6222041
GGTATCA4700.036.3851131
TATCAAC11000.034.2020032
AACGCAG11250.033.1525426
ATCAACG11750.032.0188983
CAACGCA11650.032.0001565
TCAACGC11750.031.7278164
ACGCAGA12100.031.1064267
GTATAAA553.418396E-431.092731
CGCAGAG12550.030.2770428
AGAGTAC12450.030.24527411
CAGAGTA12400.030.09116610
GGCGATC604.8132625E-429.3281857
GCAGAGT12950.028.8131629
GAGTAAG605.6903024E-428.501671
TACAGTA605.6903024E-428.501672
TTGAAAC500.00676938628.35418962
TGATTAA756.005876E-527.6908239
AGTACGG9450.027.53061913
Produced by FastQC (version 0.11.5)