FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757089

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757089
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences381771
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTCGC359.4122667E-442.40637668
GGTATCA4800.041.6203271
GTATCAA11800.038.8229331
TATCAAC13300.034.6670272
CCGGTTT400.002614764534.4444128
ACGGTTC400.002628244234.4083188
ATCAACG13700.033.4036943
TCAACGC13750.033.0319864
CAACGCA13900.032.6755265
AACGCAG14550.031.215796
ACGCAGA15500.029.3025677
ATTAGAG605.500486E-428.6735973
AGAGTAC16100.027.99682811
CGCAGAG16400.027.6944988
TAGGTTC500.00782956127.5266554
CGTCAGA756.265423E-527.5266535
CAGAGTA16500.027.3181210
GCAGAGT17000.026.7170459
CTTATAC658.728248E-426.4956971
TACGGGG6200.024.9737815
Produced by FastQC (version 0.11.5)