FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757090

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757090
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences381771
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5020.13149243918474687No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA4850.046.0051421
GTATCAA12600.042.2275541
GTATAGG655.657039E-736.9674451
TATCAAC15150.035.3326342
TCAACGC15550.034.203094
CAACGCA15800.033.879085
ATCAACG15700.033.8763123
AACGCAG16150.033.3704386
ACGCAGA16300.032.865677
ACGGGCT2200.031.21037316
GTATGTA553.3503462E-431.2062871
CGCAGAG17200.031.1459568
AGAGTAC17300.030.9659211
CAGAGTA18100.029.78698510
TACGGGC3950.029.55883615
GCAGAGT18650.028.908559
CCCATAT500.007156313428.03831556
AGTACGG14100.027.52103813
GAGTACG14650.026.7222312
GTGGTAT1950.026.405321
Produced by FastQC (version 0.11.5)