FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005757091

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757091
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1873707
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC63730.3401278855231901No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAGATGTCACGACCCAAATATAGTAGA58000.30954679680440966No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG56550.3018081268842994No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT53750.2868644884178796No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAGATGTCACGACCCAAATATAGTAGACAGTAATAAT53700.2865976377309793No Hit
CTCTTAGCCTTCATGAGTGTCCCCACCATGGCCTGGATGATGCTTCTCCT49410.263701848794929No Hit
CTCATCTACAGCACAAACACTCGCTCTTCTGGGGTCCCTGCTCGCTTCTCTGGCTCCATCCTTGGGAACAAAG44030.23498871488445097No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC43440.23183987677902682No Hit
GTAATAATCAGATTCATCATCTGCCTGGGCCCCCGTGATGGTGAGGGCAG42210.22527534988127812No Hit
GTAGATGAGCGTGCGTGGAGCCTGGCCTGGGGTCTTCTGATACCAGCTGG42200.22522197974389804No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA41780.2229804339739351No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG41170.2197248555937508No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC40800.21775016051068818No Hit
GTGGTGACCCAGGAGCCATCGCTCTCAGTGTCCCCTGGAGGGACAGTCACACTCACTTGTGGCTCGAGCTCTGG40480.21604231611452593No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG39800.21241314677268108No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT38070.2031801130059289No Hit
GATTATTACTGTCTACTATATTTGGGTCGTGACATCTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGT35980.1920257542934941No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC34550.18439382464814402No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG34270.18289946080150205No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC33230.1773489665139747No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG31800.1697170368686246No Hit
ATATTTGGGTCGTGACATCTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCC31270.16688841958748085No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC30110.16069748365139266No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC30050.16037726282711223No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA29300.15637450252360693No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT29050.1550402490891052No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG28930.15439980744054435No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG28870.15407958661626392No Hit
CCCCAGAAGAGCGAGTGTTTGTGCTGTAGATGAGCGTGCGTGGAGCCTGG28020.1495431249389579No Hit
GGATGATGCTTCTCCTCGGACTCCTTGCTTATGGATCAGGAGTGGATTCTCAGACTGTGGTGACCCAGGAGCCAT27880.14879594301563692No Hit
CTACTAGTTACTACCCCAGCTGGTATCAGAAGACCCCAGGCCAGGCTCCACGCACGCTCATCTACAGCACAAACA27370.14607406600925332No Hit
GTCTCTACTAGTTACTACCCCAGCTGGTATCAGAAGACCCCAGGCCAGGC26820.14313870845334942No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC26540.14164434460670744No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA26430.14105727309552668No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAGATGTCACGAC25060.133745564274457No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG24980.13331860317541644No Hit
CTTCTGGGGTCCCTGCTCGCTTCTCTGGCTCCATCCTTGGGAACAAAGCTGCCCTCACCATCACGGGGGCCCAGG24650.1315573886418741No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA24050.12835518039906987No Hit
CTTCATGAGTGTCCCCACCATGGCCTGGATGATGCTTCTCCTCGGACTCCTTGCTTATGGATCAGGAGTGGATT24040.1283018102616898No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT23850.12728777765146845No Hit
GTATCAACGCAGAGTACGGGAGCTGGGAAGCCTGAGAACTCTTAGCCTTC23670.1263271151786272No Hit
GGCCTGGATGATGCTTCTCCTCGGACTCCTTGCTTATGGATCAGGAGTGG23360.124672640919845No Hit
CTACTATATTTGGGTCGTGACATCTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGG23180.12371197844700371No Hit
CTTGTGGCTCGAGCTCTGGCTCAGTCTCTACTAGTTACTACCCCAGCTGGTATCAGAAGACCCCAGGCCAGGCTC23110.12333838748534323No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA22700.12115021185276033No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC21990.11736093209877532No Hit
TTCTCAGACTGTGGTGACCCAGGAGCCATCGCTCTCAGTGTCCCCTGGAG21980.11730756196139523No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC21150.11287784055884938No Hit
CATCTACAGCACAAACACTCGCTCTTCTGGGGTCCCTGCTCGCTTCTCTGGCTCCATCCTTGGGAACAAAGCTGC21090.11255761973456896No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGC21040.1122907690476686No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC20940.1117570676738679No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT20430.1090351906674843No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA20100.10727397613394198No Hit
GTGATGGTGAGGGCAGCTTTGTTCCCAAGGATGGAGCCAGAGAAGCGAGC19860.10599309283682028No Hit
ATATAGTAGACAGTAATAATCAGATTCATCATCTGCCTGGGCCCCCGTGA19710.10519254077611923No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG19710.10519254077611923No Hit
GATCAGGAGTGGATTCTCAGACTGTGGTGACCCAGGAGCCATCGCTCTCAGTGTCCCCTGGAGGGACAGTCACA19570.10444535885279824No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG19360.10332458596781673No Hit
CCCCAGGCCAGGCTCCACGCACGCTCATCTACAGCACAAACACTCGCTCT19340.1032178456930566No Hit
GCCTGGGCCCCCGTGATGGTGAGGGCAGCTTTGTTCCCAAGGATGGAGCC19280.10289762486877618No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGG19230.10263077418187583No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT18870.10070944923619328No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC18820.10044259854929292No Hit
GTCTACTATATTTGGGTCGTGACATCTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGG18760.1001223777250125No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)