FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005757094

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757094
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences609672
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC19350.3173837735700508No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC18090.2967169231980475No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17770.29146819929404666No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT16130.2645684892860423No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA14090.23110787439803698No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12840.2106050466480337No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC12130.19895944048603184No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG11660.19125037725203062No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG11440.18764187956803002No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG11320.1856736081040297No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT11210.1838693592620294No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG11190.18354131401802937No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT11130.1825571782860292No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT10630.1743560471860279No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA10030.16451468986602633No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT9900.16238239578002597No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT9810.16090619218202573No Hit
CCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCGTG9610.15762573974202523No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC9320.15286908370402444No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCCTGGTCCCTTGCCCGAATGTC8950.14680024669002348No Hit
GGACTCCTCAGTTCACCTTCTCACCATGAGGCTCCCTGCTCAGCTCCTGG8730.1431917490060229No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG8610.14122347754202258No Hit
GTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCGTGGATAGAGATA8550.14023934181002243No Hit
GTGTATAGATCAGGAGTTGTGGAGACTGCCCTGGCTTCTGCAGGAACCAGTCCACATATATGCTTCCAGTATCT8490.13925520607802228No Hit
GTATGGAGTTTCCCCGGACATTCGGGCAAGGGACCAGGGTGGAAATCAAA8430.13827107034602212No Hit
GTCTGGGACTCCAGAGGCCCGATAGGAAAGTGTATAGATCAGGAGTTGTGGAGACTGCCCTGGCTTCTGCAGGAA8150.13367843693002138No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT8060.13220223333202114No Hit
GTAATAAACTCCAACATCCTCAGCCTCCACCCTGCTGATTTTCAGTGTGAAATCAGTGCCTGACCCACTGCCA8060.13220223333202114No Hit
GTCCCAGACAGGTTCAGTGGCAGTGGGTCAGGCACTGATTTCACACTGAA7790.12777362253802046No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACCATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTC7650.1254773058300201No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC7370.12088467241401935No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCCTGGTCCCTTGCCCGAATGTCCGGGGAAACTC7340.12039260454801927No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG7280.11940846881601912No Hit
GTTTATTACTGCATGCAACGTATGGAGTTTCCCCGGACATTCGGGCAAGGGACCAGGGTGGAAATCAAACGAA7200.1180962878400189No Hit
CATATATGCTTCCAGTATCTCTATCCACGAGGCTCTGACTAGACCTGCAG7180.11776824259601884No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC7120.11678410686401869No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC7110.11662008424201867No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT7070.11596399375401856No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC6970.1143237675340183No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT6810.11169940558201787No Hit
GGACCAGGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC6770.11104331509401777No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA6550.10743481741001719No Hit
ACTCCATACGTTGCATGCAGTAATAAACTCCAACATCCTCAGCCTCCACC6490.10645068167801704No Hit
CCATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCTGCATGCAGTGAGGATATTGTGATGA6280.10300620661601648No Hit
CCACTGTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCTGCAG6200.10169402564001627No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG6170.1012019577740162No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)