FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757095

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757095
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1091915
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATAGCGGCTGCAC11210.10266366887532455No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGAT11020.10092360669099701No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA32400.044.0985341
TCGTATG1200.043.283840
GGTATCA12500.039.9373361
TCAACGC39300.035.9874884
ATCAACG39450.035.850653
CAACGCA39850.035.4907955
AACGCAG40400.035.0928086
CTCGTAT1400.034.61268639
TATCAAC41000.034.4953232
ACGCAGA42500.033.3588077
CGTATGC1500.032.3349641
CGCAGAG44450.032.0502058
AGAGTAC44750.031.75990111
CAGAGTA45400.031.38099110
GCAGAGT47300.030.1204419
ACTACGG605.4601254E-428.7156130
GAGTACG38400.028.67747512
ACGGGTA2050.028.53627816
AGTACGG38700.028.45516613
TATACAC3150.027.310633
Produced by FastQC (version 0.11.5)