FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757097

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757097
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1824328
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAATCAGCCTCATCTTCAGACTGGAGCCCACTGATGGCCAGGGAGG84810.46488350779026577No Hit
GATTATTACTGTGCAGCATGGGATGACAGCCTGAACGGCCATTGGGCGTTCGGCGGAGGGACCAAGCTGACCGTC80280.4400524467091444No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC57950.31765121184348427No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACGCCCA56480.30959345030060387No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACGCCCAATGGCCGTTC47010.2576839252590543No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGTCAACAAGG45340.24852986962870713No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC44720.24513135795756025No Hit
CCTCTGAGGAGCTTCAAGTCAACAAGGCCACACTGGTGTGTCTCATAAGT41560.22780991137558598No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC41110.22534324967878583No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT39840.2183817822233721No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT38480.21092698242859836No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG38300.2099403177498783No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC36660.20095070623265113No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG35570.19497590345595747No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG34580.1895492477229972No Hit
GCCATTGGGCGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCC34040.18658925368683701No Hit
CCCAATGGCCGTTCAGGCTGTCATCCCATGCTGCACAGTAATAATCAGCC33560.18395814787691686No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA32930.18050482150139668No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGTCAACAAGGCCACACTGGTGT30420.16674633070368924No Hit
CCCTTGAGGGCCGCTGATTATTACTATAAATGAGGAGTTTGGGGGCCGAT30070.1648278160506225No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC30000.16444411312000912No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC29810.16340263373691574No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT28950.15868856916080878No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAG28910.15846931034331546No Hit
GCATGGGATGACAGCCTGAACGGCCATTGGGCGTTCGGCGGAGGGACCAA28640.15698931332523539No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA28570.15660561039462204No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC28350.15539968689840863No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG28330.15529005748966196No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG27870.15276858108848848No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC27630.1514530281835284No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG27210.14915081059984828No Hit
ATCCTGGGAACTGCTGGTACCAGTTTATAGTATTTCTTCCGATGTTGGAGCTGCTTCCAGAACAAGACATGGTGA26930.14761599887739485No Hit
GGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCA26030.14268267548379457No Hit
CACCAGTGTGGCCTTGTTGACTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG24950.13676268741147424No Hit
CCTCAAGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCAGT24750.1356663933240075No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGACTTGAAGCTCCTCAGAGGA24620.13495380216715414No Hit
CTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTC24070.13193899342662066No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA22800.12497752597120694No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGTCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC22740.12464863774496691No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACGCCCAATGGCCGTTCA22720.12453900833622024No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC22600.1238812318837402No Hit
GGCCATCAGTGGGCTCCAGTCTGAAGATGAGGCTGATTATTACTGTGCAG22560.12366197306624686No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT22160.1214693848913134No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC22040.12081160843883337No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCAGTCTGAAGATG21960.12037309080384667No Hit
GGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGT21780.11938642612512662No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA21760.11927679671637995No Hit
ATCCCATGCTGCACAGTAATAATCAGCCTCATCTTCAGACTGGAGCCCAC21710.11900272319451327No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC21540.11807087322016656No Hit
GTCTGAAGATGAGGCTGATTATTACTGTGCAGCATGGGATGACAGCCTGA21520.11796124381141988No Hit
GCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCA21280.1166456909064598No Hit
GGATGACAGCCTGAACGGCCATTGGGCGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGC21080.11554939681899308No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGTCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGG20760.11379532627904632No Hit
GATTATTACTATAAATGAGGAGTTTGGGGGCCGATCCTGGGAACTGCTGG20490.11231532926096621No Hit
TCTTCAGACTGGAGCCCACTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTCAGGGACC20420.11193162633035286No Hit
GTAATAATCAGCGGCCCTCAAGGGTCCCTGACCGATTCTCTGGCTCCAAG20340.11149310869536619No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACGC20270.11110940576475282No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC20230.11089014694725949No Hit
GTGCAGCATGGGATGACAGCCTGAACGGCCATTGGGCGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGC19820.1086427440679527No Hit
ACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC19730.10814941172859267No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC19420.10645015589301923No Hit
ATGCTGCACAGTAATAATCAGCCTCATCTTCAGACTGGAGCCCACTGATG19230.10540867650992586No Hit
GTTTGGGGGCCGATCCTGGGAACTGCTGGTACCAGTTTATAGTATTTCTT18830.10321608833499239No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT18530.10157164720379229No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA18500.10140720309067229No Hit
GTCTTGTTCTGGAAGCAGCTCCAACATCGGAAGAAATACTATAAACTGGTACCAGCAGTTCCCAGGATCGGCCCC18460.10118794427317894No Hit
AGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGT18340.10053016782069892No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16650.046.2514721
GTATCAA42900.041.991931
TATCAAC48950.036.712552
ATCAACG52150.034.5915953
TCAACGC52600.034.2303354
CAACGCA53000.033.9719925
AACGCAG54450.033.193536
TGGTATC5300.031.7677022
ACGCAGA57150.031.6253327
AGAGTAC58050.031.19506511
CGTATGC2000.031.15974641
CGCAGAG58250.031.0281148
TCGTATG1900.030.94846540
CAGAGTA59900.030.28897510
GCAGAGT60950.029.6536129
AGTACGG46450.028.03696413
GAGTACG47250.027.78043612
GCCAGAG46600.027.26660270
TACGGGG18350.026.96447615
AGGGGGG4400.026.38581770
Produced by FastQC (version 0.11.5)