FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757099

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757099
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1152087
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTTGGGCTGACCTAGGACGGTCATCTTGGTCCCTCCGCCGAAAATTATAAGATCACTACTCCTATCCCACAC34360.29824136545243546No Hit
CCTATGTCCTGAGTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACG33000.28643670139494676No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC30600.26560494129349604No Hit
GACCTAGGACGGTCATCTTGGTCCCTCCGCCGAAAATTATAAGATCACTA29170.2531926842330484No Hit
GGATTACCTGTGGGGGTAACAACATTGGAAGTAAAAGTGTGCACTGGTACCAGCAGAAGCCAGGCCAGGCCC27760.24095402517344608No Hit
CTCCTATCCCACACCTGACAGTAATAGTCGGCCTCATCCCCGGCTTCGAC27470.23843685416118748No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT25170.21847308406396393No Hit
GTAATATACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA25050.21743149605889137No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCATCTTGGTCCCTC23920.20762320901112505No Hit
GCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCCTATGTCCTGAGT23330.20250206798618506No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT23320.20241526898576237No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC22110.19191258993461432No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG22050.19139179593207803No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG21940.19043700692742824No Hit
CCCCAGGACAGACGGCCAGGATTACCTGTGGGGGTAACAACATTGGAAGT21760.1888746249198194No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT21190.18392708189572488No Hit
GGGTGGCCGTGTTCCCAGAATTGGAGCCAGACAATCGCTCAGGGATCCCTGAGGGCCGTTTACTATCCTCATAGA21110.1832326898923432No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT20410.17715675986275342No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC20280.17602837285725817No Hit
GTATATTACTGTGCGAAACAGACGGACGTGGGAGATTACAATTTTTGGAGTGGTTATCCGGGGCACTTGGATTA20140.17481318685134023No Hit
GTCCTGAGTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACGGCCAG20050.17403199584753581No Hit
GCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGA19620.17029963882935925No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC19590.1700392418280911No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT18740.16266132679216067No Hit
ACACAGGGGCCTGGCCTGGCTTCTGCTGGTACCAGTGCACACTTTTACTT18580.1612725427853973No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG18450.16014415577990204No Hit
CTACGCAGACTCCGTGAAGGGCCAATTCACCATCTCCAGAGACAATTCCAAGAACACACTGTATCTGCAAATGAA17850.15493621575453936No Hit
GTATCAACGCAGAGTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTT17660.15328703474650787No Hit
CCTCTCTCACTGCACAGGCTCTGTGACCTCCTATGTCCTGAGTCAGCCAC17640.15311343674566244No Hit
CCCCTGTGTTGGTCGTCTATGAGGATAGTAAACGGCCCTCAGGGATCCCT17570.15250584374270346No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG17400.15103026073551737No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT17390.15094346173509465No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG17290.15007547173086755No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG17230.14955467772833128No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC16690.14486753170550487No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTG16660.14460713470423675No Hit
GGATAGGAGTAGTGATCTTATAATTTTCGGCGGAGGGACCAAGATGACCGTCCTAGGTCAGCCCAAGGCTGCCCC16590.14399954170127777No Hit
GTAATAGTCGGCCTCATCCCCGGCTTCGACCCTGCTGATGGTCAGGGTGGCCGTGTTCCCAGAATTGGAGCCAGA16570.14382594370043233No Hit
CGGCCACCCTGACCATCAGCAGGGTCGAAGCCGGGGATGAGGCCGACTAT16440.14269755669493708No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC16250.1410483756869056No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA16140.14009358668225577No Hit
GGCTCTGTGACCTCCTATGTCCTGAGTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACGGCCAGGATTAC16060.13939919467887407No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG15930.13827080767337882No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCCTATGTCCTGAGTCAGC15770.13688202366661545No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA15740.1366216266653473No Hit
GCACAGGCTCTGTGACCTCCTATGTCCTGAGTCAGCCACCCTCGGTGTCA15740.1366216266653473No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT15730.1365348276649246No Hit
ATCCCACACCTGACAGTAATAGTCGGCCTCATCCCCGGCTTCGACCCTGC15650.1358404356615429No Hit
CTCCTGTGCAGCCTCTGGATTCACATTTAGCAGTTATGCCATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGG15590.13531964165900665No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT15190.1318476816420982No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC15120.13124008863913925No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA14700.12759453062138537No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT14390.12490376160828132No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT14150.12282058559813626No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG14060.12203939459433184No Hit
GTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCCTA14020.12169219859264101No Hit
CTCACTGCACAGGCTCTGTGACCTCCTATGTCCTGAGTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACGG14000.12151860059179559No Hit
CTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAGCTCATGGCATAACTGC14000.12151860059179559No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC13830.1200430175846095No Hit
GCCCTGGGATCTTCAGGTGTTTTCATTTGGTGATCAGGACTGAACAGAGA13690.11882783157869153No Hit
GTCTGCGTAGTGTGTGGCACCACCACTACCACTAATAGTTGAGACGCACT13450.11674465556854648No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA13440.11665785656812377No Hit
GGCCAGGCCCCTGTGTTGGTCGTCTATGAGGATAGTAAACGGCCCTCAGG13370.11605026356516478No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG13240.11492187655966954No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT13160.11422748455628785No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC13070.11344629355248344No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC13030.1130990975507926No Hit
GTGTTGGTCGTCTATGAGGATAGTAAACGGCCCTCAGGGATCCCTGAGCG13010.11292549954994718No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA12870.11171031354402924No Hit
GATCAGGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGC12640.10971393653430687No Hit
GGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTC12580.10919314253177059No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT12400.1076307605241618No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG12310.1068495695203574No Hit
CAGTAATAGTCGGCCTCATCCCCGGCTTCGACCCTGCTGATGGTCAGGGT12120.1052003885123259No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC12050.10459279550936691No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCATC11950.10372480550513981No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA11820.10259641849964456No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCATCTTGGTCCCTCCGCCGAAAATTATAAGATCA11620.10086043849119034No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG11570.10042644348907678No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAG11540.10016604648780865No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTACC150.002366832268.9168859
CTATAAC200.00739418951.6876644
GTGGTAT2350.042.5601271
GTATCAA17000.040.9803121
GTAGTTT650.001178058140.51989770
TCGTATG602.855213E-740.34861840
TGGTATC2400.040.201522
GGTATCA7000.037.4445231
CGTATGC655.3331314E-737.25786241
TATCAAC19300.036.2438552
ATCAACG19900.035.3242343
TCAACGC20000.034.975324
CAACGCA20150.034.7149545
TAACGGC400.002585705134.52887736
AACGCAG20300.034.4584476
TTATACA807.1699105E-834.4584432
TATACAC851.2212877E-732.4314773
GAGTACT4150.032.38263712
AGAGTAC21750.032.3196411
CAGAGTA21750.032.16121310
Produced by FastQC (version 0.11.5)