FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005757100

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757100
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1152087
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTTGGGCTGACCTAGGACGGTCATCTTGGTCCCTCCGCCGAAAATTATAAGATCACTACTCCTATCCCACAC34780.3018869234701893No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC30750.26690692629983676No Hit
CCTATGTCCTGAGTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACG29300.2543210712385436No Hit
GACCTAGGACGGTCATCTTGGTCCCTCCGCCGAAAATTATAAGATCACTA27470.23843685416118748No Hit
GGATTACCTGTGGGGGTAACAACATTGGAAGTAAAAGTGTGCACTGGTACCAGCAGAAGCCAGGCCAGGCCC26180.22723978310665774No Hit
GGGTGGCCGTGTTCCCAGAATTGGAGCCAGACAATCGCTCAGGGATCCCTGAGGGCCGTTTACTATCCTCATAGA25210.21882028006565477No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT24970.2167371040555097No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT23640.2051928369992891No Hit
GTAATATACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA23420.20328325898998945No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT23230.20163407798195798No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG22990.19955090197181288No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCATCTTGGTCCCTC22560.19581854495363632No Hit
GCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCCTATGTCCTGAGT22280.1933881729418004No Hit
CTCCTATCCCACACCTGACAGTAATAGTCGGCCTCATCCCCGGCTTCGAC22060.19147859493250075No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG20430.17733035786359885No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC20080.17429239284880393No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT19780.17168842283612262No Hit
CCCCAGGACAGACGGCCAGGATTACCTGTGGGGGTAACAACATTGGAAGT19430.16865045782132773No Hit
GCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGA19340.16786926681752334No Hit
GTCCTGAGTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACGGCCAG19290.16743527181540976No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC19230.1669144778128735No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC18940.16439730680061487No Hit
GTATCAACGCAGAGTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTT18880.16387651279807863No Hit
GTATATTACTGTGCGAAACAGACGGACGTGGGAGATTACAATTTTTGGAGTGGTTATCCGGGGCACTTGGATTA18450.16014415577990204No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCCTATGTCCTGAGTCAGC18410.15979695977821118No Hit
CCCCTGTGTTGGTCGTCTATGAGGATAGTAAACGGCCCTCAGGGATCCCT17990.15615140176045733No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG17450.15146425573763092No Hit
CCTCTCTCACTGCACAGGCTCTGTGACCTCCTATGTCCTGAGTCAGCCAC17130.14868668772410415No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT17100.14842629072283606No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA16850.14625631571226824No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT16610.1441731397021232No Hit
GGATAGGAGTAGTGATCTTATAATTTTCGGCGGAGGGACCAAGATGACCGTCCTAGGTCAGCCCAAGGCTGCCCC16310.14156916968944186No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG16230.14087477768606016No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC16120.13991998868141037No Hit
ACACAGGGGCCTGGCCTGGCTTCTGCTGGTACCAGTGCACACTTTTACTT15960.13853120467464697No Hit
GTAATAGTCGGCCTCATCCCCGGCTTCGACCCTGCTGATGGTCAGGGTGGCCGTGTTCCCAGAATTGGAGCCAGA15810.1372292196683063No Hit
CTACGCAGACTCCGTGAAGGGCCAATTCACCATCTCCAGAGACAATTCCAAGAACACACTGTATCTGCAAATGAA15770.13688202366661545No Hit
GGCTCTGTGACCTCCTATGTCCTGAGTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACGGCCAGGATTAC15640.1357536366611202No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG15390.13358366165055244No Hit
GTGTTGGTCGTCTATGAGGATAGTAAACGGCCCTCAGGGATCCCTGAGCG15390.13358366165055244No Hit
GGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTC15220.13210807864336635No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTG15150.13150048564040737No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG15060.13071929463660295No Hit
GTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCCTA14970.12993810363279856No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG14850.12889651562772603No Hit
CGGCCACCCTGACCATCAGCAGGGTCGAAGCCGGGGATGAGGCCGACTAT14780.12828892262476704No Hit
CTCCTGTGCAGCCTCTGGATTCACATTTAGCAGTTATGCCATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGG14610.12681333961758096No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC14590.12663974161673555No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT14320.12429616860532235No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC14300.12412257060447691No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA14260.12377537460278606No Hit
GCACAGGCTCTGTGACCTCCTATGTCCTGAGTCAGCCACCCTCGGTGTCA14250.12368857560236338No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA14240.12360177660194066No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT14220.12342817860109523No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT14060.12203939459433184No Hit
GCCCTGGGATCTTCAGGTGTTTTCATTTGGTGATCAGGACTGAACAGAGA13970.12125820359052744No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG13920.1208242085884139No Hit
ATCCCACACCTGACAGTAATAGTCGGCCTCATCCCCGGCTTCGACCCTGC13470.11691825356939188No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC13460.11683145456896919No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG13270.11518227356093766No Hit
GTCTGCGTAGTGTGTGGCACCACCACTACCACTAATAGTTGAGACGCACT13190.11448788155755597No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA13110.11379348955417429No Hit
GGCCAGGCCCCTGTGTTGGTCGTCTATGAGGATAGTAAACGGCCCTCAGG13090.11361989155332887No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT13060.11335949455206075No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG12940.11231790654698821No Hit
GGGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTT12890.11188391154487465No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC12850.11153671554318381No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT12780.11092912254022481No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT12760.11075552453937941No Hit
CTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAGCTCATGGCATAACTGC12710.11032152953726584No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT12630.10962713753388416No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG12410.1077175595245845No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA12190.10580798151528487No Hit
CTCACTGCACAGGCTCTGTGACCTCCTATGTCCTGAGTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACGG12080.10485319251063506No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTA11960.10381160450556252No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT11910.10337760950344896No Hit
GTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT11900.10329081050302624No Hit
GATCAGGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGC11780.10224922249795372No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA11680.10138123249372662No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC11660.10120763449288118No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)