FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757101

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757101
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1572711
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCAC35930.22845901122329534No Hit
CTCCTGCACTGGGAGCAGCTCCAACATCGGGGCAGCTTATGATGTACACTGGTACCAGCGCCTTCCAGGAACAGC28920.18388629570213472No Hit
GTCATAGGACTGGCAGTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAG27590.17542956080297015No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC27250.1732676887234845No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACATGAACACTC27190.17288618188592816No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC24870.15813458416708476No Hit
GTAATACACAGCCGTGTCCTCAGCTCTCAGACTGCTCATTTGAAGATACA22650.14401883117750178No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT21850.13893207334341784No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG20990.1334638086717776No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG19980.1270417769062466No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC19070.1212555898699761No Hit
GCTTATGATGTACACTGGTACCAGCGCCTTCCAGGAACAGCCCCCAAACTCCTCATCTATAGTAACAGCAATCGG17990.11438846679396278No Hit
GTGTATTACTGTGTGAAAGCGGATTACTATGGTTCGGGGAGGTTTTACTA17950.11413412890225857No Hit
GTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTCAGGGACCCCTGAGGGCCGATTG17890.11375262206470228No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT17820.11330753075421995No Hit
GTCCTATGACAGCAGCCTGAGTGTTCATGTGGTATTCGGCGGAGGGACCA17230.10955604685158303No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC16860.10720342135331921No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG16800.1068219145157629No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG16740.1064404076782066No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT16570.10535947163846378No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC16380.10415136665286884No Hit
GGCCATCACTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCCAGT16300.10364269086946044No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT16190.1029432616672739No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG15950.1014172343170487No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCGT200.007004038452.40202357
GGTATCA14650.043.765531
GTATCAA35050.040.2246441
ACGATCG350.001272523739.89319656
TGGTATC5300.037.700462
TATCAAC40350.035.090762
ATCAACG41150.034.4922753
TCAACGC41700.034.1199574
CAACGCA42800.033.2430465
AACGCAG43850.032.3684656
TCGACTA450.004656567730.6216213
AGAGTAC46650.030.49854311
CAGAGTA47350.030.12042210
CGCAGAG47600.029.8898538
GCAGAGT49100.028.9767239
AGTACGG37750.027.74197213
CGACTAG500.007791458627.55945814
ACGGGTA1653.6379788E-1227.14275416
GAGTACG38850.026.95648412
ACGCAGA54500.026.1688427
Produced by FastQC (version 0.11.5)