FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757102

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757102
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1572711
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCAC30420.19342396664104214No Hit
CTCCTGCACTGGGAGCAGCTCCAACATCGGGGCAGCTTATGATGTACACTGGTACCAGCGCCTTCCAGGAACAGC28960.18414063359383892No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC26880.17091506322522065No Hit
GTCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCACCATCTCCTG23010.14630787220283956No Hit
GTCATAGGACTGGCAGTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAG21620.1374696304661187No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG21500.1367066167910061No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACATGAACACTC20510.1304117539713272No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC19770.12570650297479957No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT18930.12036540724901142No Hit
GTAATACACAGCCGTGTCCTCAGCTCTCAGACTGCTCATTTGAAGATACA18800.11953880910097278No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG18620.11839428858830389No Hit
GTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCAC17830.11337111522714599No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT17720.11267168602495944No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC17680.11241734813325525No Hit
GCTTATGATGTACACTGGTACCAGCGCCTTCCAGGAACAGCCCCCAAACTCCTCATCTATAGTAACAGCAATCGG17340.11025547605376956No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17330.11019189158084353No Hit
GTCCTATGACAGCAGCCTGAGTGTTCATGTGGTATTCGGCGGAGGGACCA17300.11000113816206539No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG17270.10981038474328723No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT17260.10974680027036117No Hit
GTGTATTACTGTGTGAAAGCGGATTACTATGGTTCGGGGAGGTTTTACTA16980.1079664350284318No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT16570.10535947163846378No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC16450.1045964579633512No Hit
GGCCATCACTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCCAGT16030.10192591010045711No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA36000.040.449791
GGTATCA15200.039.8609351
TGGTATC5950.038.757292
TATCAAC41200.035.0871122
ATCAACG42650.034.0556343
TCAACGC42500.034.013864
CAACGCA43300.033.3854265
AACGCAG43950.032.892726
CGCAGAG46500.031.4680358
CAGAGTA47550.030.62931810
AGAGTAC47700.030.6051811
GCAGAGT49550.029.4625039
GGTAACG500.007389622327.86009652
AGTACGG42000.026.39696713
ACGCAGA56350.026.0862927
GAGTACG42800.026.06445912
ACGGGTA1359.646101E-925.50188316
TTAACGG1501.035005E-925.31690635
TTTAACG1651.1459633E-1025.09887334
TACGGGG22250.023.52053615
Produced by FastQC (version 0.11.5)