FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757103

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757103
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1245737
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAATCAGCCTCATCCTCAGACTGGAGCCCACTGATGGCCAGGGAGG61970.4974565257353679No Hit
GATTATTACTGTGCAGCATGGGATGACAGCCTGAACGGCCATTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTC54220.43524435735632805No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCA47580.38194257696447964No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC40390.32422573946186073No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC39380.31611808913117295No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAATGGCCGTTC36550.2934006134521171No Hit
CCCAATGGCCGTTCAGGCTGTCATCCCATGCTGCACAGTAATAATCAGCC34460.27662339643118894No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT33630.2699606738822079No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG32850.26369932016147873No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC32360.2597659056446104No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGTCAACAAGG31620.25382564698648274No Hit
CCTCTGAGGAGCTTCAAGTCAACAAGGCCACACTGGTGTGTCTCATAAGT29020.23295446791738544No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC28650.22998433858832162No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT28540.22910132716616752No Hit
GCCATTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCC28470.22853941080661488No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG27810.2232413422736902No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC27330.21938820152247224No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAG26520.21288602650479196No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG26500.2127254789734912No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC25940.20823014809707024No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA25000.20068441412593507No Hit
CCCTTGAGGGCCGCTGATTATTACTATAAATGAGGAGTTTGGGGGCCGTTCCTGGGAACTGCTGGTACCAGTTTA24970.20044359282898397No Hit
GGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCA24730.19851702245337496No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC24430.19610880948386375No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC23890.1917740261387436No Hit
GTCTGAGGATGAGGCTGATTATTACTGTGCAGCATGGGATGACAGCCTGA23450.1882419804501271No Hit
TCCTCAGACTGGAGCCCACTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTCAGGGACC23290.18695760019972113No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGTCAACAAGGCCACACTGGTGT22620.18157925790114607No Hit
CACCAGTGTGGCCTTGTTGACTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG22390.1797329612911875No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT22370.17957241375988672No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGACTTGAAGCTCCTCAGAGGA21940.17612064183692064No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG21900.17579954677431914No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG21470.17234777485135305No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG21180.1700198356474922No Hit
GCATGGGATGACAGCCTGAACGGCCATTGGGTGTTCGGCGGAGGGACCAA21080.16921709799098847No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA21060.16905655045968773No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACAC19010.15260042850136105No Hit
GGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGT19010.15260042850136105No Hit
CCTCAAGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCAGT18590.14922893034404533No Hit
GCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCA18380.14754318126538746No Hit
CTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTC18110.14537578959282738No Hit
GTAATACACAGCCGTGTCCTCGGGAGTCACAGAGTTCAACTGCAGGGAGA17930.14393086181112064No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC17800.14288730285766577No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAATGGCCGTTCA17150.13766950809039147No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCAGTCTGAGGATG16780.13469937876132765No Hit
ACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC16750.1344585574643765No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA16630.133495272276572No Hit
GTTTGGGGGCCGTTCCTGGGAACTGCTGGTACCAGTTTACAGTATTTCTT16590.1331741772139705No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC16460.13213061826051567No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC16270.13060541671315856No Hit
ATCCCATGCTGCACAGTAATAATCAGCCTCATCCTCAGACTGGAGCCCAC16180.1298829528223052No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGTCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC16110.1293210364627526No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT16090.12916048893145182No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA16090.12916048893145182No Hit
GATTATTACTATAAATGAGGAGTTTGGGGGCCGTTCCTGGGAACTGCTGG16070.12899994140015109No Hit
GTAATAATCAGCGGCCCTCAAGGGTCCCTGACCGATTCTCTGGCTCCAAG15890.12755501361844435No Hit
GGCCATCAGTGGGCTCCAGTCTGAGGATGAGGCTGATTATTACTGTGCAG15700.12602981207108724No Hit
CTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTC15600.1252270744145835No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA15580.12506652688328276No Hit
GGATGACAGCCTGAACGGCCATTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGC15350.12322023027332414No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC15180.12185557625726778No Hit
AGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGT15160.12169502872596703No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGTCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGG14790.1187248993969032No Hit
ATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTG14620.11736024538084684No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGACTTGAAG14610.11727997161519647No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTC14600.11719969784954609No Hit
ATGCTGCACAGTAATAATCAGCCTCATCCTCAGACTGGAGCCCACTGATG14560.11687860278694459No Hit
CCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGG14520.11655750772434309No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC14410.11567449630218898No Hit
GTGCAGCATGGGATGACAGCCTGAACGGCCATTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGC14260.11447038981743336No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG14100.1131860095670274No Hit
GTGTGGCCTTGTTGACTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC14020.1125438194418244No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT13720.11013560647231317No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC13620.10933286881580943No Hit
CTCTTGTTCTGGAAGCAGCTCCAACATCGGAAGAAATACTGTAAACTGGTACCAGCAGTTCCCAGGAACGGCCCC13620.10933286881580943No Hit
GTGCCAGACTTGGAGCCAGAGAATCGGTCAGGGACCCTTGAGGGCCGCTG13270.10652328701804634No Hit
GCTTGGTCCCTCCGCCGAACACCCAATGGCCGTTCAGGCTGTCATCCCATGCTGCACAGTAATAATCAGCCTCAT13000.10435589534548624No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT12940.103874252751584No Hit
GCCCTCAAGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAG12780.10258987250117801No Hit
AGACACACCAGTGTGGCCTTGTTGACTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGC12640.10146603978207279No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11200.047.974361
GTATCAA29700.042.2131351
TATCAAC33750.037.3321722
TGGTATC3150.037.1573142
ATCAACG35500.035.4918563
CAACGCA36100.035.0926865
TCAACGC36050.034.9503674
CTAGTAC400.002624214934.425163
AACGCAG36750.034.3783266
ACGCAGA38450.032.8570257
AGAGTAC39250.032.0144911
CAGAGTA39550.031.77164810
CGCAGAG39750.031.6958548
GCAGAGT40500.031.1113939
CGTATGC756.0450566E-527.67900741
GCTAATC756.185971E-527.58552633
AGTACGG32050.027.49937213
AGTACTT13850.027.34347713
GAGTACT14000.027.05051212
GAGTACG32500.027.01267812
Produced by FastQC (version 0.11.5)