FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757106

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757106
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1235851
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT36780.2976086923099953No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG29950.24234313036118432No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC26120.21135233940013806No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC25220.20406990810380865No Hit
GTATGATAATCTCCCTCCGGACACTTTTGGCCAGGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT25110.20317983316759058No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC23690.19168977490004863No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC23440.1896668773177349No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG22700.18367910047408628No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT22650.18327452095762353No Hit
CTTTTGGCCAGGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCA22500.1820607824082353No Hit
GGCCAAAAGTGTCCGGAGGGAGATTATCATACTGTTGACAGTAATATGTTGCAATATCTTCAGGCTGCAGGCTG21990.17793407134031528No Hit
GTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGGACATCAGCAA20340.1645829472970447No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCCTGGCCAAAAGTG20050.1622363861015608No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG19500.1577860114204706No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGGACATCAGCAA19340.1564913569677898No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC18230.14750969170231687No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT18120.14661961676609883No Hit
AGTCAGGACATCAGCAACAATTTAAATTGGTATCAGCAGAAATCAGGGAA17800.14403030786073726No Hit
TTATCATACTGTTGACAGTAATATGTTGCAATATCTTCAGGCTGCAGGCTGCTGATGGTGAAAGTAAAATCTGTC17760.14370664424756704No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT16770.1356959698216047No Hit
GTAATATGTTGCAATATCTTCAGGCTGCAGGCTGCTGATGGTGAAAGTAAAATCTGTCCCAGATCCACTTCCA16740.13545322211172706No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT15710.12711888407259453No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG15580.12606697732979139No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT15250.12339675252113726No Hit
CAGTAATATGTTGCAATATCTTCAGGCTGCAGGCTGCTGATGGTGAAAGTAAAATCTGTCCCAGATCCACTTCCA15230.12323492071455217No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG14970.12113110722894588No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG14630.11837996651699921No Hit
CAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGGCAT14530.11757080748407372No Hit
AACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTC14480.11716622796761099No Hit
CCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCT14480.11716622796761099No Hit
ATATTACTGTCAACAGTATGATAATCTCCCTCCGGACACTTTTGGCCAGGGGACCAAGGTGGAGATCAAACGAA14120.11425325544907922No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGG14060.11376776002932393No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA13830.11190669425359528No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG13510.10931738534823374No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA13170.10656624463628707No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA13120.10616166511982432No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC13100.10599983331323923No Hit
GATCTACGATGCATCCAATTTGGAAACAGGGGTCCCATCAAGGTTCAGTGGAAGTGGATCTGGGACAGATTTTA12930.1046242629572659No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG12600.10195403814861176No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG12550.10154945863214904No Hit
GGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCT12550.10154945863214904No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT12390.10025480417946823No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA28600.043.0441251
GTGGTAT3800.040.721691
TATCAAC32700.037.3256342
TCAACGC34350.035.232424
ATCAACG34500.035.1788983
CAACGCA34800.034.7768335
AACGCAG35750.033.951616
AGAGTAC37950.032.00023711
CGCAGAG38750.031.4270948
ACGCAGA38800.031.3840547
CAGAGTA39400.030.82256110
TAGTTAG850.00468823530.57657670
GCAGAGT40150.029.9897429
CGATTGT900.00619943728.8778870
CCCGTAT500.00784422627.52098
AGTACGG32650.026.86873613
GAGTACG33400.026.574412
CCGATTA700.001281072724.8143246
ACGGGTA2250.024.46500416
GAGTACT13400.024.38984312
Produced by FastQC (version 0.11.5)