FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757108

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757108
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1622974
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTAAGTAGTTCATATTGTTGGAGCTGTATAAAAGACT54820.33777497359785186No Hit
GTAATACACGGCCGTGTCCGCGGCAGTCACAGAGCTCAGGTTCAGGGAGA40650.25046611960511994No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC38420.2367259118137444No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT32680.20135874018930677No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT32360.19938705117888517No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT31800.19593659541064737No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC31050.1913154492924717No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC30910.19045283535041227No Hit
GTCTCTGGTGGCTCCATCAGCAGTGGTAGTTACTACTGGAGCTGGATCCG30390.18724884070847717No Hit
CTCCTAAGCTGCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCT30360.18706399486375014No Hit
GTATAAAAGACTCTGGCTGGACTTGCAGCTGATGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA29310.18059439029830424No Hit
GTGTATTACTGTGCGAGCGCGCGCCACTATGATCACTTTTGGGGGAGTTATCGGACCCTATTTGACTACTGGGGC26990.16629964497274757No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC26920.16586833800171782No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC26340.16229465167032867No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT25300.15588666238645846No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGCTGGTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA24160.14886252028683145No Hit
GTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCGGATTCCCGGGT24100.14849282859737742No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG23940.1475069840921666No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC23550.14510398811071526No Hit
CTTTTATACAGCTCCAACAATATGAACTACTTAGCTTGGTACCAGCAGAA22930.1412838406530234No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACACCTGTGGTTCTTCCTCCTGCTGGTGGCAGCTCCCAGATGGGTC22830.14066768783726663No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT22560.1390040752347234No Hit
CTTTTGGCCAGGGGACCAGGCTGGAGATCAAACGAACTGTGGCTGCACCA22050.13586169587436397No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC22010.13561523474806125No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG21660.13345869989291265No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT21180.13050116637728024No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG20500.1263113272301343No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT20410.12575678969595322No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG20290.1250174063170451No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT19910.12267602561716946No Hit
GTCAGGGACCCCGGATTCCCGGGTAGATGCCCAGTAAATGAGCAGCTTAGGAGGCTGTCCTGGTTTCTGCTGGTA19530.12033464491729381No Hit
CACCTGCACTGTCTCTGGTGGCTCCATCAGCAGTGGTAGTTACTACTGGA19520.12027302963571813No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC19330.11910233928578029No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA19070.11750034196481274No Hit
CTATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGGTGATGGTGAGAGTGAAATCTGTC18810.11589834464384519No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG18740.11546703767281545No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG18150.11183173605985061No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG17930.11047619986518577No Hit
CAGTAATACACGGCCGTGTCCGCGGCAGTCACAGAGCTCAGGTTCAGGGAGAACTGGTTCTTAGATGTGTCAA17440.10745705106797769No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG17210.10603989959173715No Hit
TCTTCAGCCTGCAGGCTGGTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACC17050.10505405508652634No Hit
GTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTC16880.10400659529973987No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT16840.10376013417343717No Hit
GTAAATGAGCAGCTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTA16620.10240459797877231No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAGCTGCAAGTCCAGCCAGAGTCTTTTATA16450.10135713819198582No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT4550.049.952791
TGGTATC4600.048.65072
GTATCAA26800.040.3480031
AACTGAT13650.038.0497670
GGTATCA11650.036.9497341
TCAACGC31100.034.2083134
ATCAACG31150.034.0428733
TATCAAC31650.033.8314172
CAACGCA31400.033.771835
AACGCAG32250.033.101366
CGCAGAG35400.030.0743758
CAGAGTA35600.029.90541810
GCAGAGT36300.029.2338169
ACGCAGA38850.027.398617
AGTACGG37100.026.46746413
GAGTACG37950.025.96543912
CAACTGA13800.025.19233969
GTGCTAC1401.41608325E-824.5979651
AAGTATT1350.001429852424.34970570
GTGTTAC851.4616283E-424.3085771
Produced by FastQC (version 0.11.5)