FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757109

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757109
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1658255
Sequences flagged as poor quality0
Sequence length20-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA49900.039.907611
GGTATCA21350.038.406751
GTGGTAT7350.034.687491
TCAACGC57450.034.5201574
ATCAACG57700.034.4899253
TATCAAC58950.033.7585952
CAACGCA59150.033.6444445
AACGCAG61000.032.6805236
ACGCAGA63850.031.2757237
CGCAGAG66450.029.9483688
AGAGTAC66600.029.83011811
TGGTATC8750.029.1181622
CAGAGTA68800.029.02638410
GCAGAGT70700.028.1480759
GCGTAAG500.007811504427.54503411
GAGTACG47850.026.62399112
AGTACGG47900.026.52431713
TACGGGT10900.025.5865615
ATACGCC1001.5889067E-524.101186
ACGGGTA3900.023.8370516
Produced by FastQC (version 0.11.5)