FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757110

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757110
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1658255
Sequences flagged as poor quality0
Sequence length20-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16710.10076857901830538No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25500.037.336831
GTATCAA53700.037.2517431
GTGGTAT8850.035.3423961
ATCAACG60800.032.439583
TCAACGC60750.032.3531574
CAACGCA61100.032.1678285
TATCAAC62200.031.8199142
AACGCAG64150.030.7473966
ACGCAGA67700.029.0931027
TGGTATC10600.028.5261862
CGCAGAG69550.028.5195088
AGAGTAC70250.028.08937311
CAGAGTA73000.027.12539710
GCAGAGT74200.026.547729
AGTACGG52100.025.33786413
GAGTACG52000.025.18825512
GTACGGG56650.022.93867514
AATAGCG4300.022.1143462
TACGGGG21750.021.81006615
CGAACCT4500.021.74677753
Produced by FastQC (version 0.11.5)