FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757113

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757113
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1476989
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACAGCCGTGTCCTCAGCTCTCAGACTGTTCATTTCCAAATAAA28520.19309554776643562No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA18470.12505170993148898No Hit
CTCCAGACCCTTGCCTGGAGCCTGGCGGACCCAGTGCATGCCAGAATTTC17940.12146332843372563No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT17920.12132791781116854No Hit
GTGTATTACTGTGCGAAAGTGGAGGGTTATTATGGTTCGGGAGAGCTTGA16960.11482820792842736No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT16150.10934407771486448No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT15630.1058234015283797No Hit
GTATAATAACTGGCCTCCGTCCGCGCTCTTCGGCCCTGGGACCAAAGTGG15550.10528175903815128No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT15120.10237043065317346No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13150.042.0674631
GTATCAA31100.039.883481
TATCAAC36000.034.241972
ATCAACG37050.033.178873
TCAACGC37350.033.096244
CAACGCA37850.032.7497565
AACGCAG38200.032.3598066
AGAGTAC41100.030.1641411
CGCAGAG41800.029.4095388
GCAGAGT42750.028.8372949
ACGCAGA42850.028.768057
CAGAGTA46200.026.83433510
AGTACGG35350.025.84150713
GAGTACG36150.025.26963412
CGATTCA9600.024.96644870
TACGGGG19350.023.78125215
TGCACAG13650.023.72813670
TCGTATG902.1151417E-423.01858740
GAGTACT14400.022.89466712
GTACGGG39750.022.89466714
Produced by FastQC (version 0.11.5)